Bìol. Tvarin, 2019, volume 21, issue 3, pp. 65–73

INFLUENCE OF TRADITIONAL AND GENETICALLY MODIFIED SOYBEANS ON THE FUNCTIONAL STATUS OF THE EXCRETORY SYSTEM IN THREE GENERATIONS OF LABORATORY ANIMALS

N. Omelchenko1, G. Dronyk2, I. Kukovska3, A. Mikheev3

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1National Technical University “Kharkiv Polytechnic Institute”, Chernivtsi Faculty,
203а Holovna str., Chernivtsi 58018, Ukraine

2Bukovyna State Agricultural Research Station NAAS,
21a Bohdana Kryzhanivskoho str., Chernivtsi 58026, Ukraine

3Higher State Educational Establishment of Ukraine “Bukovynian State Medical University”,
2 Teatralna sq., Chernivtsi 58002, Ukraine

A significant distribution and widespread use of genetically modified soybean based food in human nutrition and animal feeding may cause the pathological changes in their organisms. In order to understand clearly the long-term effects of exposure to living organisms of genetically modified plants and food products containing GMOs, long-term multilateral studies are required. The aim of our work was to study the effect of native and genetically modified soybeans on the functional state of the kidneys of the studied laboratory animals. The experiment was conducted on three generations of Wistar female rats divided into three groups — control, 1st experimental, and 2nd experimental. The control animals received standard vivarium feed. The experimental groups received a standard diet with 35 % protein replacement in the beans native and transgenic soybeans, respectively. After 12 months of consumption of the experimental diet, the functional status of the animal kidneys was assessed on the parameters of the excretory, ion-regulating, acid-excretion functions in conditions of spontaneous diuresis. The growth of drinking activity in the 1st and 2nd experimental groups of all three generations was revealed; the concentration and excretion of endogenous creatinine in the urine of female rats of all generations in both experimental groups increased; an increase in serum creatinine and urea levels in female rats that used GM soy compared to animals that consumed native soy and animals from the control group while maintaining these changes over three generations was noted. The absence of significant changes in the indicators of the kidneys regulating function in sexually mature female rats of three generations in both experimental groups was revealed; the absence of noticeable shifts in electrolyte levels in the serum of three generations of female rats in the experimental groups of animals that consumed traditional and transgenic soybeans.

Keywords: TRADITIONAL SOYBEAN, GENETICALLY MODIFIED SOYBEAN, RATS, KIDNEY, RENAL FUNCTION

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