Bìol. Tvarin, 2019, volume 21, issue 3, pp. 47–55

LABORATORY AND PRODUCTION TESTING OF THE POSTBIOTIC’S EFFECTIVENESS

M. Kucheruk, L. Vygowska

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National University of Life and Environmental Sciences of Ukraine,
15 Heroyiv Oborony str., Kyiv 03041, Ukraine

Organic poultry farming is a perspective branch of livestock breeding. The production of high quality and safe chicken meat requires prophylactic alternative to antibiotics, since they are prohibited from using by law. The purpose of the research was to test the developed postbiotics based on metabolites of symbiotic bacteria in vitro (under microbiological laboratory conditions) and in vivo (under certified organic farming). Laboratory testing of the bactericidal activity of the finished product in two concentrations of bacteriucin Nizyn (0.05 and 0.1 g). The test culture of Escherichia coli, Yersinia enterocolitica, Listeria ivanovii, Staphylococcus aureus Bacillus cereus microorganisms was used. The manufacturing test of the drug concerned the use of chicken broilers to prevent the emergence of bacterial etiology. It has been established that test variants no. 1 and no. 2 of postbiotics containing 0.05 g and 0.10 g of Nizyn showed a pronounced inhibitory effect in vitro on growth of test cultures of Escherichia coli ATCC 25922 (F50), Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC no. 25923, respectively. Postbiotic variant no. 1 was active against Listeria ivanovii ATCC 19119, variant no. 2 with respect to Yersinia enterocolitica ATCC 23715. In the study of production test the postbiotic effect in vivo on chicken broilers, the possibility of its use for the prevention of infections caused by pathogenic bacteria was established. Due to its bactericidal properties, the microbiocenosis of the digestive canal is corrected, and immunity and health and productivity improves. We managed to reduce the death of bird by 31 % compared to the control group of chickens who did not receive prophylactic drugs. Unlike antibiotics, the components of postbiotics (bacteriocin and lactic acid) are completely cleaved and excreted from the body without accumulation in residual amounts in the body of the bird.

Keywords: CHICKENS, ORGANIC PRODUCE, ORGANIC POULTRY FARMING, POSTBIOTIC, BACTERIOCIN, BACTERICIDAL PROPERTIES

  1. Iebba V., Totino V., Gagliardi A., Santangelo F., Cacciotti F., Trancassini M., Mancini C., Cicerone C., Corazziari E., Pantanella F., Schippa S. Eubiosis and dysbiosis: the two sides of the microbiota.  The New Microbiologica: official journal of the Italian Society for Medical Virology (SIVIM), 2016, vol. 39, issue 1, pp. 1–12. Available at: https://www.researchgate.net/publication/296481400_Eubiosis_and_Dysbiosis_The_Two_Sides_of_the_Microbiota (accessed Mar 10 2019).
  2. Kucheruk M. D. Sanitary and hygienic aspects of correction of microendocology of intestines of animals. The World of Science and Innovation “Scientific World”, 2016, vol. 2, issue 10, pp. 32–35. (in Ukrainian)
  3. Kucheruk M. D., Zasekin D.A. Microendoecology intestines of animals. Nutraceutics. Kyiv, Scientific-production enterprise “Interservis” LLC, 2013, 344 p. (in Ukrainian)
  4. Liu Y., Yang X., Xin H., Chen S., Yang C., Duan Y., Yang X. Effects of a protected inclusion of organic acids and essential oils as antibiotic growth promoter alternative on growth performance, intestinal morphology and gut microflora in broilers. Animal Science Journal, 2017, vol. 88, issue 9, pp. 1414–1424. DOI: 10.1111/asj.12782.
  5. McFarland L. V. Antibiotic-associated diarrhea: epidemiology, trends and treatment. Future Microbiology, 2008, vol. 3, issue 5, pp. 563–578. DOI: 10.2217/17460913.3.5.563.
  6. Meghrous J., Euloge R., Junelles A. M., Ballongue J., Petitdemange H. Screening of Bifidobacterium strains for bacteriocin production. Biotechnology Letters, 1990, vol. 12, issue 8, pp. 575–580. DOI: 10.1007/BF01030755.
  7. Otchenashko V. V. Use of lactic acid in livestock. Kyiv, 2012, 46 p. (in Ukrainian)
  8. Sologub Yu. O. Organic production as a component of ecological and food safety of Ukraine. Organic production and food safety. Zhytomyr, ZNAMEU, 2017, pp. 398–401. (in Ukrainian)
  9. Zasekin D., Prus V., Reva O. In the WTO and the EU — without antibiotics in feeds and livestock products! Veterinary Medicine of Ukraine, 2006, vol. 4, pp. 30–31. (in Ukrainian)
 
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