Bìol. Tvarin, 2019, volume 21, issue 3, pp. 9–13

DETERMINATION OF THE SECURITY CRITERION IN THE CONTENT OF CONVENTIONAL ANIMALS BY THE IMPROVED HORIZONTAL METHOD OF LISTERIA MONOCYTOGENES DETECTION

N. M. Bogatko

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National Agrarian University,
10/1 Soborna sq., Bila Tserkva 09117, Ukraine, This email address is being protected from spambots. You need JavaScript enabled to view it.

It is particularly important for market operators — such as producers of meat from slaughtered animals, wholesale bases, agroindustrial markets, supermarkets, etc. — to determine one of the safety criteria, the presence of Listeria monocytogenes during the production of meat raw materials, its transportation, storage and marketing. The absence of this pathogenic microorganism indicates the proper sanitary and hygienic status of the business entities, the observance of personal hygiene for the production and circulation of beef, pork, mutton, goat meat. The conducted researches have revealed that the colonies of Listeria monocytogenes were detected in 24±2 hours and were small in size (1.5–2.0 mm), gray-green or olive-green, sometimes with black halo; in 46±2 hours — green with a flask center and black halo. These colonies were detected in the following samples of slaughter meat: in 2 samples of beef, 3 samples of pork and 1 sample of goat meat sold in the agroindustrial markets, in 2 samples of pork and 1 sample of beef sold in supermarkets. In beef, pork, mutton during production at facilities and storage at wholesale bases, as well as in mutton and goat meat realized on the agromarkets and in supermarkets, the characteristic colonies of Listeria monocytogenes were not detected. The improved horizontal detection method of Listeria monocytogenes is economical in terms of using nutrient media, it is simple to implement, and its results provide specific qualitative indices of the color and size of Listeria monocytogenes colonies, this method can be used in combination with other methods for determining the safety of slaughter animals. The reliability of the developed improved horizontal method for detecting Listeria monocytogenes in slaughtered animals was 99.8 %. The improved horizontal method for detecting Listeria monocytogenes in slaughter meat can be applied throughout the food chain for defining safety criteria for the production of safe meat, its transportation, storage and sale in production laboratories of meat processing plants, and enterprises for the implementation and storage of meat of slaughtered animals (in shops, supermarkets, wholesale bases, refrigerators, etc.), as well as in state laboratories of veterinary medicine and their departments.

Keywords: MICROBIOLOGICAL CRITERION, SAFETY, SLAUGHTERED ANIMALS’ MEAT, BEEF, PORK, MUTTON, GOAT MEAT, LISTERIA MONOCYTOGENES

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© 2016 Institute of Animal Biology

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