DYNAMICS OF INTENSITY OF THE PROTEIN OXIDATIVE MODIFICATION PROCESSES AND THE STATE OF THE ANTIOXIDANT PROTECTION SYSTEM IN CHICKEN-BROILERS UNDER THE EFFECT OF BPS-44 AND THE DRIED PREPARATIONS OF SACCHAROMYCES CEREVISIAE

N. N. Romanovich1, B. M. Kurtyak1, M. S. Romanovich1, O. I. Vishchur2, D. I. Mudrak2, I. O. Matiukha2

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1Lviv National University of Veterinary Medicine and Biotechnologies named after Stepan Gzhytsky,
50 Pekarska str., Lviv 79010, Ukraine

2Institute of Animal Biology NAAS,
38 V. Stus str., Lviv 79034, Ukraine

The purpose of the research was to find out the effects of the feeding of chicken broilers of Ross-308 cross with standard feed of BPS-44 and 1 and 2 % yeast Saccharomyces cervisisia on the intensity of the processes of oxidative modification of proteins (OMP) and the state of the antioxidant protection system (APS).

Experiments were conducted in 4 groups of 100 broiler chickens in each according to the scheme: the chickens of control group were fed with standard feed (SC) according to the existing norms recommended for the Ross-308 cross. The first experimental group in addition to the SC received a probiotic BPS-44, made on the basis of the production strain of bacteria Bacillus subtilis ssp. subtilis 44-p, dose 0.21 g/kg, second experimental group — 1 % yeast Saccharomyces cerevisiae; the third experimental group — 2 % yeast Saccharomyces cerevisiae. The material for conducting biochemical studies was the blood of chickens, which was taken from the subclavian vein at different age intervals: 27, 34 and 41 days of age.

The results of the conducted studies showed that the probiotic drugs under investigation caused a revised effect on the OMP and APS activity. In particular, the inhibitory effect was determined as a probiotic based on the strain of Bacillus subtilis 44 and yeast Saccharomyces cerevisiae on the intensity of the accumulation of products of oxidative modification of proteins in plasma of chickens. This effect was more pronounced on the 41st day of the chicken experiment, which used 2 % yeast Saccharomyces cerevisiae. However, as evidenced by the results of experimental studies, in the action of 1 and 2 % of yeast Saccharomyces cerevisiae in red blood cells of chickens, an increase in superoxide dismutase (SOD) activity and an increase in the content of reduced glutathione (RG) was observed. At the same time, when studying the activity of another enzyme of the system of antioxidant protection — glutathione peroxidase (GP), it should be noted that significant changes in age dynamics, as well as the influence of investigational drugs were not found.

Thus, based on the experimental studies, it is possible to state the positive effect of the studied drugs, and especially the yeast of Saccharomyces cerevisiae on the intensity of OMP and the activity of the system of antioxidant protection of broiler chickens.

Keywords: BROILERS CHICKENS, BPS-44, YEAST SACCHAROMYCES CEREVISIAE, ANTIOXIDANT PROTECTION

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