Bìol. Tvarin, 2019, volume 21, issue 1, pp. 48–54

DYNAMICS OF INTENSITY OF THE PROTEIN OXIDATIVE MODIFICATION PROCESSES AND THE STATE OF THE ANTIOXIDANT PROTECTION SYSTEM IN CHICKEN-BROILERS UNDER THE EFFECT OF BPS-44 AND THE DRIED PREPARATIONS OF SACCHAROMYCES CEREVISIAE

N. N. Romanovich1, B. M. Kurtyak1, M. S. Romanovich1, O. I. Vishchur2, D. I. Mudrak2, I. O. Matiukha2

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1Lviv National University of Veterinary Medicine and Biotechnologies named after Stepan Gzhytsky,
50 Pekarska str., Lviv 79010, Ukraine

2Institute of Animal Biology NAAS,
38 V. Stus str., Lviv 79034, Ukraine

The purpose of the research was to find out the effects of the feeding of chicken broilers of Ross-308 cross with standard feed of BPS-44 and 1 and 2 % yeast Saccharomyces cervisisia on the intensity of the processes of oxidative modification of proteins (OMP) and the state of the antioxidant protection system (APS).

Experiments were conducted in 4 groups of 100 broiler chickens in each according to the scheme: the chickens of control group were fed with standard feed (SC) according to the existing norms recommended for the Ross-308 cross. The first experimental group in addition to the SC received a probiotic BPS-44, made on the basis of the production strain of bacteria Bacillus subtilis ssp. subtilis 44-p, dose 0.21 g/kg, second experimental group — 1 % yeast Saccharomyces cerevisiae; the third experimental group — 2 % yeast Saccharomyces cerevisiae. The material for conducting biochemical studies was the blood of chickens, which was taken from the subclavian vein at different age intervals: 27, 34 and 41 days of age.

The results of the conducted studies showed that the probiotic drugs under investigation caused a revised effect on the OMP and APS activity. In particular, the inhibitory effect was determined as a probiotic based on the strain of Bacillus subtilis 44 and yeast Saccharomyces cerevisiae on the intensity of the accumulation of products of oxidative modification of proteins in plasma of chickens. This effect was more pronounced on the 41st day of the chicken experiment, which used 2 % yeast Saccharomyces cerevisiae. However, as evidenced by the results of experimental studies, in the action of 1 and 2 % of yeast Saccharomyces cerevisiae in red blood cells of chickens, an increase in superoxide dismutase (SOD) activity and an increase in the content of reduced glutathione (RG) was observed. At the same time, when studying the activity of another enzyme of the system of antioxidant protection — glutathione peroxidase (GP), it should be noted that significant changes in age dynamics, as well as the influence of investigational drugs were not found.

Thus, based on the experimental studies, it is possible to state the positive effect of the studied drugs, and especially the yeast of Saccharomyces cerevisiae on the intensity of OMP and the activity of the system of antioxidant protection of broiler chickens.

Keywords: BROILERS CHICKENS, BPS-44, YEAST SACCHAROMYCES CEREVISIAE, ANTIOXIDANT PROTECTION

  1. Bokun A. A., Derevyanenko S. V. Application of probiotics in livestock breeding. Veterinary Medicine, 2002, pp. 94–97. (in Ukrainian).
  2. Davies K. J. A. Oxidative stress, antioxidant defenses, and damage removal, repair, and replacement systems. IUBMB Life, 2000, vol. 50, issue 4–5, pp. 279–289. https://doi.org/10.1080/713803728
  3. Dubinina E. E., Salnikova L. A., Efimova L. F. Activity and isoenzyme spectrum of superoxide dismutase of human red blood cells and blood plasma. Laboratory Practice, 1983, no. 10, pp. 30–33. (in Russian)
  4. Goryachkovsky O. M. Determining the level of restored glutathione in red blood cells (method E. Butler, O. Dubon, B. Kelly, 1963). Clinical biochemistry. Odesa, Astroprint, 1998, pp. 370–372.
  5. Harms N. K., Bertel-Harms R. M., Bruer-Kleis D. Enzyme substitution therapy with yeast Saccharomies cerevisie in congenital sucroseisomaltase deficiency. The New England Journal of Medicine, 1987, vol. 316, pp. 1306–1309. https://doi.org/10.1056/NEJM198705213162104
  6. Kalitka V. V., Donchenko G. V. Antioxidant system and peroxide oxidation of lipids in chickens for postnatal ontogenesis. Ukrainian biochemistry journal, 1995, no. 2, pp. 80–85. (n Ukrainian)
  7. Klaenhammer T. R., Kleerebezem M., Kopp M. V., Rescigno M. The effect of probiotics and prebiotics on the immune system. Nature Reviews Immunology, 2012, vol. 12, pp. 728–734. https://doi.org/10.1038/nri3312
  8. Kotsyumbas I. Ya., Rozhko M. S., Kushnir I. M. Application of probiotics in veterinary medicine. Veterinary Medicine of Ukraine, 2003, no 10, pp. 15–17. (in Ukrainian)
  9. Kovalchuk Ya. Ya. Influence of feeding of biomass of yeast of Saccharomices cerevisii on the antioxidant status of pigs. Scientific and technical bulletin of the Institute of Animal Biology of NAAS and DNDKI of veterinary preparations and feed supplements, 2006, vol. 7, no. 1–2, pp. 186–188. (in Ukrainian)
  10. Landis G. N., Tower J. Superoxide dismutase evolution and life span regulation. Mechanisms of Ageing and Development, 2005, vol. 126, issue 3, pp. 365–379. https://doi.org/10.1016/j.mad.2004.08.012
  11. Meschyshen I. F. Method of determination of oxidative modification of plasma proteins (serum). Bukovyna honey bulletin, 1998, vol. 2, issue 1, pp. 156–158. (in Russian)
  12. Meschyshen I. F., Poloviy V. P., Ryabov G. Ya., Azi Yu. M., Dorokhov S. I. Mechanism of oxidative modification of proteins, Oxidative modification of blood plasma proteins in patients in critical states, etc. Anesthesiology and reanimatology, 2000, no. 2, pp. 72–75. (in Russian)
  13. Meshchyshen I. F., Poloviy V. P. The mechanism of oxidation of protein modification. Bukovyna honey bulletin, 1999, vol. 3, no. 1, pp. 196–205. (in Russian)
  14. Mishra V., Shah C., Mokashe N., Chavan R., Yadav H., Prajapati J. Probiotics as Potential Antioxidants: A Systematic Review. Journal of Agricultural and Food Chemistry, 2015, vol. 63, pp. 3615–3626. https://doi.org/10.1021/jf506326t
  15. Moin V. M. A simple and specific method for determining the activity of glutathione peroxidase in erythrocytes. Laboratory Practice, 1986, no. 12, pp. 724–727.
  16. Pentilyuk S. I. Modern feed biologics. Animal breeding of Ukraine, 2005, no. 6, pp. 25–27. (in Ukrainian)
  17. Petryankin F. P., Ivanova Yu. I. Use of probiotics in livestock and poultry farming. The state of the problem of veterinary, hygiene and ecology in livestock: Materials of international scientific-practice conf., Cheboksary, 2004, pp. 450–453. (in Russian)
  18. Simonenko M. M. Peroxide oxidation of lipids and indicators of protein metabolism in chicken broilers under different systems of maintenance and respiratory diseases. Autoref. of PhD thesis in agricultural science, Kyiv, 2005, 18 p. (in Ukrainian)
  19. Stegniy B. T., Truskova T. Yu. Probations in animal husbandry and some aspects of designing and application. Probiotics — XXI century: Biology. Medicine. Practice: Materials Between People. scientific-practical conference, Ternopil, 2004, 234 p. (in Ukrainian)
  20. Tsekhimstrenko S. I., Polischuk V. M. Age features of the system of antioxidant protection of blood of ostriches. Ukrainian biochemistry Magazine, 2010, vol. 82, issue 5. (in Ukrainian)

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