Bìol. Tvarin, 2019, volume 21, issue 1, pp. 27–33

THE AGE FEATURES OF RAT ERYTHROCYTES

L. V. Koba1, O. E. Nipot2, O. O. Shapkina2, A. E. Zhujkova1, V. A. Bondarenko1

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1V. N. Karazin Kharkiv National University,
4 Svobody sq., Kharkiv 61022, Ukraine

2Institute for Problems of Cryobiology and Cryomedicine NAS of Ukraine,
23 Pereyaslavska str., Kharkiv 61016, Ukraine

In this work, age features of sensitivity of the 1- and 12-month rats’ erythrocytes to hypertonic environment conditions after their temperature modification at +49 °C and/or incubation in sucrose solutions were investigated.

The obtained data shows that temperature modification of cells increases their sensitivity to hypertonic stress. In this case, the red cells of the 1-month rats are more sensitive. Pre-incubation in hypertonic sucrose from 0.4 to 0.8 M significantly increases sensitivity to hypertonic shock in both 1 and 12-month-old rats. Increasing the incubation time in sucrose also increases the sensitivity of erythrocytes in both age groups to the influence of the hypertonic solution. Additionally, the cells of 1-month-old animals became more sensitive. It is believed that denaturation of ankyrin at a temperature of 49 °C leads to partial detachment of the spectrin from the membrane and increases the sensitivity of erythrocytes of 1-month-old rats to hypertonic stress. It has been shown that for native cells, the erythrocytes’ degree of sensitivity different aged rats to hypertonic stress does not depend on previous incubation in solutions of sucrose, whereas after the previous temperature modification at 49 °C, erythrocytes of 1-month rats become more sensitive. Consequently, low ionic strength equally affects the cells of rats of different ages, which demonstrates the complete formation of the spectrine-actin complex in 1-month-old rats. Removal of the spectrum-ankyrin interaction via thermodenaturation leads to an increase in the sensitivity exlusively in 1 month-old animals. This indicates the presence of additional links in erythrocytes of adult animals that appear during the process of organim’s maturation as a substitute for the spectrin-ankyrin bonds.

Thus, the erythrocytes osmotic stability (cells of the erythrocytic population) of rats in the early stages of ontogenesis is determined by the state of the structural-functional complex spectrin-ankyrin, while mature cells demonstrate a more diverse complex of plasma membrane-cytoskeleton ligaments, which determine their resistance to selected modifications.

Keywords: RATS ERYTHROCYTE, AGE FEATURES, THERMODENATURATION, HYPERTONIC SHOCK, SUCROSE SOLUTIONS, ANKYRIN, SPECTRIN, CYTOSKELETON

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