Bìol. Tvarin, 2019, volume 21, issue 1, pp. 27–33


L. V. Koba1, O. E. Nipot2, O. O. Shapkina2, A. E. Zhujkova1, V. A. Bondarenko1

This email address is being protected from spambots. You need JavaScript enabled to view it.

1V. N. Karazin Kharkiv National University,
4 Svobody sq., Kharkiv 61022, Ukraine

2Institute for Problems of Cryobiology and Cryomedicine NAS of Ukraine,
23 Pereyaslavska str., Kharkiv 61016, Ukraine

In this work, age features of sensitivity of the 1- and 12-month rats’ erythrocytes to hypertonic environment conditions after their temperature modification at +49 °C and/or incubation in sucrose solutions were investigated.

The obtained data shows that temperature modification of cells increases their sensitivity to hypertonic stress. In this case, the red cells of the 1-month rats are more sensitive. Pre-incubation in hypertonic sucrose from 0.4 to 0.8 M significantly increases sensitivity to hypertonic shock in both 1 and 12-month-old rats. Increasing the incubation time in sucrose also increases the sensitivity of erythrocytes in both age groups to the influence of the hypertonic solution. Additionally, the cells of 1-month-old animals became more sensitive. It is believed that denaturation of ankyrin at a temperature of 49 °C leads to partial detachment of the spectrin from the membrane and increases the sensitivity of erythrocytes of 1-month-old rats to hypertonic stress. It has been shown that for native cells, the erythrocytes’ degree of sensitivity different aged rats to hypertonic stress does not depend on previous incubation in solutions of sucrose, whereas after the previous temperature modification at 49 °C, erythrocytes of 1-month rats become more sensitive. Consequently, low ionic strength equally affects the cells of rats of different ages, which demonstrates the complete formation of the spectrine-actin complex in 1-month-old rats. Removal of the spectrum-ankyrin interaction via thermodenaturation leads to an increase in the sensitivity exlusively in 1 month-old animals. This indicates the presence of additional links in erythrocytes of adult animals that appear during the process of organim’s maturation as a substitute for the spectrin-ankyrin bonds.

Thus, the erythrocytes osmotic stability (cells of the erythrocytic population) of rats in the early stages of ontogenesis is determined by the state of the structural-functional complex spectrin-ankyrin, while mature cells demonstrate a more diverse complex of plasma membrane-cytoskeleton ligaments, which determine their resistance to selected modifications.


  1. Bereznyakova A. I., Jemela O. D. Deformability of the erythrocytes membrane in rats of different age in hypoxia. Physiological journal, 2013, vol. 59, issue 3, pp. 72–77. (in Ukrainian)
  2. Filho W. J., Lima C. C., Paunksnis M. R. R., Silva A. A., Perilhão M. S., Caldeira M., Bocalini D., Souza de R. R. Reference database of hematological parameters for growing and aging rats. The Aging Male, 2018, vol. 21, issue 2, pp. 145–148. https://doi.org/10.1080/13685538.2017.1350156
  3. Giuliani A. L., Graldi G., Veronesi M., Previato A., Simoni M., Bergamini C., BertiG. Binding of anti-spectrin antibodies to red blood cells and vesiculation in various in vivo and in vitro ageing conditions in the rat. Experimental Gerontology, 2000, vol. 35, issue 8, pp. 1045–1059. https://doi.org/10.1016/S0531-5565(00)00173-X
  4. Ivanov I. T., Paarvanova B. K., Ivanov V., Smuda K., Bäumler H., Georgieva R. Effects of heat and freeze on isolated erythrocyte submembrane skeletons. General Physiology and Biophysics, 2017, vol. 36, issue 2, pp. 155–165. https://doi.org/10.4149/gpb_2016046
  5. Kaneko J. J., Harvey J. W., Bruss M. L. Clinical Biochemistry of Domestic Animal. San Diego (CA), Academic Press, 2008, 928 p. https://doi.org/10.1016/B978-0-12-370491-7.X0001-3
  6. Kumar D., Rizvi S. I. Markers of oxidative stress in senescent erythrocytes obtained from young and old age rats. Rejuvenation Research, 2014, vol. 17, issue 5, pp. 446–452. https://doi.org/10.1089/rej.2014.1573
  7. McCutcheon J. E., Marinelli M. Age matters. European Journal of Neuroscience, 2009, vol. 29, issue 5, pp. 997–1014. https://doi.org/10.1111/j.1460-9568.2009.06648.x
  8. Mester A., Magyar Z., Molnar A., Somogyi V., Tanczos B., Peto K., Nemeth N. Age- and gender-related hemorheological alterations in intestinal ischemia-reperfusion in the rat. Journal of Surgical Research, 2018, vol. 225, pp. 68–75. https://doi.org/10.1016/j.jss.2017.12.043
  9. Moersdorf D., Egee S., Hahn C., Hanf B., Ellory C., Thomas S., Bernhardt I. Transmembrane potential of red blood cells under low ionic strength conditions. Cellular Physiology and Biochemistry, 2013, vol. 31, pp. 875–882. https://doi.org/10.1159/000350105
  10. Rebrova T. Y., Afanasiev S. A., Popov S. V. Age-dependent changes in Na+,K+-ATPase activity and lipid peroxidation in membranes of erythrocytes during cardiosclerosis development in rats. Bulletin of Experimental Biology and Medicine, 2016, vol. 161, issue 2, pp. 235–236. https://doi.org/10.1007/s10517-016-3384-4
  11. Repin N. V., Bobrova E. N., Repina S. V. Thermally induced transformation of mammalian red blood cells during hyperthermia. Bioelectrochemistry, 2008, vol. 73, issue 2, pp. 101–105. https://doi.org/10.1016/j.bioelechem.2008.04.017
  12. Shapkina O. A., Semionova E. A., Orlova N. V., Synchykova O. P., Shpakova N. M. Effect of glucose and partial dehydration on resistance of mammalian erythrocytes to hypertonic shock. The Animal Biology, 2015, vol. 17, issue 3, pp. 132–138. (in Ukrainian)
  13. Shnyrov V. L., Orlov S. N., Zhadan G. G., Pokudin N. I. Thermal inactivation of membrane proteins, volume-dependent Na+, K+-cotransport, and protein kinase C activator-induced changes of the shape of human and rat erythrocytes. Biomedica biochimica acta, 1990, vol. 49, issue 6, pp. 445–453.
  14. Shpakova N. M., Orlova N. V., Iershov S. S., Iershova N. A., Aleksandrova D. I Temperature and osmolarity as factors determining resistance of mammalian erythrocytes to hypertonic shock. Bulletin of problems biology and medicine, 2015, vol. 3, issue 1, pp. 242–246. (in Russian)
  15. Shpakova N. M., Orlova N. V., Nipot E. E., Shapkina O. A., Mazur A. A. Osmotic sensitivity of mammalian erythrocytes under their initial state modification. Bulletin of problems biology and medicine, 2016, vol. 3, issue 2, pp. 356–361. (in Russian)
  16. Singh S., Pandey K. B., Rizvi S. I. Erythrocyte senescence and membrane transporters in young and old rats. Archives of Physiology and Biochemistry, 2016, vol. 122, issue 4, pp. 228–234. https://doi.org/10.1080/13813455.2016.1190761
  17. Somogyi V., Peto K., Deak A., Tanczos B., Nemeth N. Effects of aging and gender on microrheology of blood in 3 to 18 months old male and female Wistar (Crl:WI) rats. Biorheology, 2018, vol. 54, issue 5–6, pp. 127–140. https://doi.org/10.3233/BIR-17148

Download full text in PDF

© 2016 Institute of Animal Biology