Bìol. Tvarin, 2018, volume 20, issue 4, pp. 74–80

QUANTITATIVE TRAIT LOCI POLYMORPHISM IN THE RESERVE POPULATION OF DUAL-PURPOSE CHICKENS

L.V. Shulika

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Institute of Animal Science NAAS,
1A Tvarynnykiv str., Kharkiv 61026, Ukraine

Studying of quantitative trait loci DNA-polymorphism of reserve chicken population is interesting for detection and preserving of selection valuable alleles and genotypes in Ukrainian chicken gene pool for the next using of them in marker-assisted selection. Thus, the aim of the study was to analyze population genetic structure of line 02 of Rhode Island Red chicken breed on mutations G2109A of myostatin gene and T+3737C and A+3971G of insulin locus.

Genotypes were determined by PCR-RFLP method. In the results of the study myostatin and insulin loci was polymorphic in investigated chicken population. In line 02 allele frequencies were: for MSTN G2109A — A=0.16, G=0.84; for INS T+3737C — C=0.74, T=0.26; for INS A+3971G — A=0.22, G=0.78. Only individuals with AG and GG genotypes were revealed in studied population on MSTN G2109A mutation. There was presence of all three possible genotypes for both mutations in insulin locus. Line 02 was in the genetic equilibrium on all investigated mutations. Heterozygosity indexes Ho and He had average values. Wright’s fixation index was point to excess of heterozygotes on MSTN G2109A and INS T+3737C mutations on levels 19 and 5 %, respectively, and to deficiency of heterozygotes on the level 18 % in the case of INS A+3971G mutation. Line 02 was characterized by average polymorphic level on studied loci. Wherein, effective allele number mean value was 1.51 for all three mutations. When compared allele frequencies distribution features in lines 02 and 38 of Rhode Island Red breed there was no reliability differences between lines on all investigated mutations.

Keywords: QUANTITATIVE TRAIT LOCI, MYOSTATIN GENE, INSULIN GENE, POLYMORPHISM, MOLECULAR GENETIC MARKER, RESTRICTION, GENETIC STRUCTURE, POPULATION, CHICKEN

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