Bìol. Tvarin, 2018, volume 20, issue 4, pp. 26–33

THE EFFECT OF SILICON COMPOUNDS ON RESISTANCE OF THE RABBITS

A. I. Ivanytska, Ya. V. Lesyk, M. M. Tsap

This email address is being protected from spambots. You need JavaScript enabled to view it.

Institute of Animal Biology NAAS,
38 V. Stus str., Lviv 79034, Ukraine

In the presented material, the results of the 14-day fertilization study and up to 20 days of lactation for the silicon citrate obtained by the method using nanotechnology, calculated from 50 μg Si/kg body weight and metasilicate of sodium in the amount of 2.5 mg Si/kg of weight body on indicators of resistance of their organism. Resistance indices were studied on the 10th day of the preparatory period and on the 20th day of lactation of the rabbits (65 days of supplements). The research has established the significant (P<0.010.001) intergroup differences in the relative content of phagocyte activity of neutrophils, bactericidal and lysozyme activity in blood of rabbits on the 20th day of lactation, which was emitted by organic and inorganic silicon citrate compounds, indicating the stimulatory effect of Silicon on cellular and humoral links of non-specific resistance of their organism.

The content of hexoses bound to proteins and ceruloplasmin in the blood of animals of the Іst and ІІnd experimental groups respectively increased by 37.7 and 22.0 % (P<0.05) and 18.2 and 13.7 % (P<0.010.001) on 65th day of the study compared with the control group. The indicated changes within the limits of physiological quantities in the blood testify to the activation of immune-physiological protection systems in the body of the rabbits during the period of increased physiological load. The less pronounced effect on the content of sialic acids in the body of the rabbits was noted by the action of metasilicate sodium, but the organic compound of silicon was marked by a stimulatory effect on their concentration.

The results of the study of the content of glycoproteins and their carbohydrate components in the blood are confirmed by a higher concentration of immunoglobulins. The use of citrate silicon resulted in a significant increase (P<0.05) of the content of immunoglobulins in the blood of the rabbits of the experimental group at 65 days of study compared with the control. This indicates the stimulatory effect of Si citrate on the synthesis of certain classes of immunoglobulins in the lymphatic system, which regulates the mechanisms of immunity.

Kerwords: RABBITS, SILICON CITRATE, SODIUM METASILICATE, RESISTANCE, IMUNOGLOBULINS, GLUCOPROTEINS

  1. Avtsyn A. P., Zhavoronkov A. A., Rish M. A., Strochkova L. S. Human trace elements: etiology, classification, organopathology. Moscow, Medicine, 1991, 496 p. (in Russian)
  2. Bissé E., Epting T., Beil A., Lindinger G., Lang H., Wieland H. Reference values for serum silicon in adults. Analytical Biochemistry, 2005, vol. 337, issue 1, pp. 130–135. https://doi.org/10.1016/j.ab.2004.10.034
  3. Boguszewska-Czubara A., Pasternak K. Silicon in medicine and therapy. Journal of Elemtology, 2011, vol. 16, issue 3, pp. 489–497. https://doi.org/10.5601/jelem.2011.16.3.13
  4. Bugaeva I. O., Egorova A. V., Zlobina O. V. Kinetics of cellular populations under influence of infra-red low-power laser irradiation. Saratov. Sci.-Med. J., 2010, vol. 6, no. 1, pp. 23–26.
  5. Chekman I. S., Ulberg Z. R., Malanchuk V. O. Nanoscience, nanobiology, nanopharmaceutics. Kyiv, Poligraphplus, 2012, 328 p. (in Ukrainian)
  6. Daoud N. M., Mahrous K. F., Ezzo O. H. Feed restriction as a biostimulant of the production of oocytes, their quality and GDF-9 gene expression in rabbit oocytes. Animal Reproduction Science, 2012, vol. 136, issue 1–2, pp. 121–127. https://doi.org/10.1016/j.anireprosci.2012.09.011
  7. De Blas C., Wiseman J. Nutrition of the Rabbit. 2nd Edition. Library of Congress Cataloging-in-Publication Data, 2010, 325 p. https://doi.org/10.1079/9781845936693.0000
  8. Eliseev A. A., Lukashin A. V. Functional Nanomaterials. Moscow, Fizmatlit, 2010, 456 p. (in Russian)
  9. García-García R. M., Rebollar P. G., Arias-Álvarez M., Sakr O. G., Bermejo-Álvarez P., Brecchia G., Gutierrez-Adan A., Zerani M., Boiti C., Lorenzo P. L. Acute fasting before conception affects metabolic and endocrine status without impacting follicle and oocyte development and embryo gene expression in the rabbit. Reproduction, Fertility and Development, 2011, vol. 23, no. 6, pp. 759–768. https://doi.org/10.1071/RD10298
  10. Gerasimov I. G. Functional heterogeneity of neutrophils. Clinical laboratory diagnostics, 2006, no. 2, pp. 34–36. (in Russian)
  11. Gordova V. S., Dyachkova I. M., Sergeeva V. E., Sapozhnikov S. P., Smorodchenko A. T. Morphofunctional Adaptation of Rat Thymus Structures to Silicon Consumption with Drinking Water. Bulletin of Experimental Biology and Medicine, 2015, vol. 158, no. 6, pp. 816–819. https://doi.org/10.1007/s10517-015-2869-x
  12. Gordova V. S., Sapozhnikov S. P., Sergeeva V. E., Karyshev P. B. The basics of biosilicification (literature review). Vestn. Chuvashsk. Gos. Univer., 2013, no. 3, pp. 401–409. (in Russian)
  13. Guinez C., Mir A.-M., Dehennaut V., Cacan R., Harduin-Lepers A., Michalski J.-C., Lefebvre T. Protein ubiquitination is modulated by O-GlcNAc glecosylation. The FASEB Journal, 2008, vol. 22, no. 8, pp. 2901–2911. https://doi.org/10.1096/fj.07-102509
  14. Jurkić L. M., Cepanec I., Pavelić S. K., Pavelić K. Biological and therapeutic effects of ortho-silicic acid and some ortho-silicic acid-releasing compounds: New perspectives for therapy. Nutrition & Metabolism (Lond.), 2013, vol. 10, no. 2, pp. 1–12. https://doi.org/10.1186/1743-7075-10-2
  15. Kim S.-Y., Kim S.-K., Choi M.-K. Effect of Silicon Supplementation on Immune Response in Male and Female Mice. The FASEB Journal, 2016, vol. 30, no. 1 suppl., pp. 34–40.
  16. Kosinov M. V., Kaplunenko V. G. Method of obtaining metal carboxylates. Nanotechnology for obtaining metal carboxylates. Patent of Ukraine for Utility Model No. 38391. IPC (2006): C07C 51/41, C07F 5/00, C07F 15/00, C07C 53/126 (2008.01), C07C 53/10 (2008.01), A23L 1/00, B82B 3/00. Publ. 2009, bull. no. 1/2009. (in Ukrainian)
  17. Kreisman L. S., Coob B. A. Infection, inflammation and carbohydrates: a Glyco-Evasion Hypothesis. Glycobiology, 2012, vol. 22, issue 8, pp. 1019–1030. https://doi.org/10.1093/glycob/cws070
  18. Müller W. E. G., Schröder H. C., Burghard Z., Pisignano D., Wang X. Silicateins — a novel paradigm in bioinorganic chemistry: enzymatic synthesis of inorganic polymeric silica. Chemistry, 2013, vol. 19, issue 19, pp. 5790–5804. https://doi.org/10.1002/chem.201204412
  19. Müller W. E. G., Schröder H. C., Wang X. The Understanding of the Metazoan Skeletal System, Based on the Initial Discoveries with Siliceous and Calcareous Sponges. Marine Drugs, 2017, vol. 15, issue 6, p. 172. https://doi.org/10.3390/md15060172
  20. Na M., Park H., Ahn M., Lee H., Chung I. Synthesis of organic-inorganic hybrid sols with nanosalica particles and organoalkokysilanes for transparent and high-thermal-resistance coating films using solgel reaction. Journal of Nanoscience and Nanotechnology, 2010, vol. 10, no. 10, pp. 6992–6995. https://doi.org/10.1166/jnn.2010.2988
  21. Official Journal of the European Union L276/33, 2010. Directive 2010/63/EU of The European Parliament and of The Council of 22 September 2010 on the protection of animals used for scientific purposes. 86/609/EC. 20.10.2010
  22. Pavlata L., Mišurová L., Pechová A., Dvořák R., Comparison of organic and inorganic forms of selenium in the mother and kid relationship in goats. Czech Journal of Animal Science, 2012, vol. 57, no. 8, pp. 361–369. https://doi.org/10.17221/6271-CJAS
  23. Plehova N. G. Bactericidal activity of phagocytes. Journal of Epidemiology, Microbiology and Immunobiology, 2006, no. 6, pp. 89–96. (in Russian)
  24. Sapozhnikov S. P., Gordova V. S. Microelements. Medicine, 2013, no. 14, pp. 3–13.
  25. Shtapenko O., Gevkan I., Dzen Y., Fedorova S., Slyvchyk Y., Syrvatka V., Matiukha I. Effect of liposomal preparate with some organic trace elements on antioxidant status and reproductive ability of female rabbits. Scientific Papers, Series D. Animal Science, 2015, vol. LVIII, pp. 221–224.
  26. Sybirna N. O., Shevtsova A. I., Ushakova G. O., Brodyak I. V., Pismenetska I. M. Fundamentals of Glycobiology. A monograph. Lviv, Lviv National University named after Ivan Franko, 2015, 492 p. (in Ukrainian)
  27. Vlizlo V. V. (ed.), Fedoruk R. S., Ratych I. B. Laboratory methods of investigation in biology, stock-breeding and veterinary. A reference book. Lviv, 2012, 764 p. (in Ukrainian)
  28. Xiccato G., Trocino A. Energy and Protein Metabolism and Requirements. In: de Blas C., Wiseman J. (ed). Nutrition of the Rabbit. 2nd edition. CABI, Wallingford, UK, 2010, pp. 83–118. https://doi.org/10.1079/9781845936693.0083
  29. Yurashhak S. V., Noreyko A. Yu. The efficiency of the production of rabbit meat when kept in a closed rabbit. The act of the problem of intensive development of livestock, Gorki, 2013, vol. 16, no. 1, pp. 322–329. (in Russian)
© 2016 Institute of Animal Biology

Search