Bìol. Tvarin, 2018, volume 20, issue 3, pp. 77–83

TRANSFUSION OF CANINE CRYOPRESERVED RED BLOOD CELLS IN TREATMENT OF BABESIOSIS

О. А. Pervushina, ONDenysova, GFGegunov, NIGladka, VOPrichodchenko, TIYakimenko

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Kharkiv state zooveterinary academy, HDZVA,
Mala Danylivka, Derhachivsky district, Kharkiv region, 62341, Ukraine

The results of the evaluation of the clinical and biochemical efficiency of dogs cryopreserved red blood cells transfusion with acute form of babesiosis are presented. Cryopreserved erythrocyte mass was obtained by rapid immersion of red blood cells in liquid nitrogen with subsequent heating in a water bath. As a cryoprotectant, a 17.5 % solution of hydroxyethyl starch 200 was used. Research studies were on three groups of animals control and two experimental (animals with acute form of babesiosis and basic drug treatment; animals with acute form of babesiosis, basic drug treatment and transfusion of cryopreserved erythrocytes).

It has been established that the application of cryopreserved erythrocyte transfusion leads to an increase in the level of hematocrit and hemoglobin, as well as the red blood cell count in 24 hours. On the 10th day these indices were close to the control values and almost twice exceed the hematological parameters in animals with basic treatment. Biochemical parameters of blood serum of dogs with babesiosis pointed out uremia, high creatinine level, and an increased activity of liver test enzymes at the first day of the disease. Ten days after the basic course of therapy in combination with hemotransfusion of cryopreserved erythrocytes, in patient dogs there is a normalization of the activity of alanine aminotransferase, the amount of bilirubin and urea. The amount of creatinine and the activity of aspartate aminotransferase and lactate dehydrogenase in this group of experimental animals return to the reference levels.

It was shown that transfusion of cryopreserved erythrocyte mass is highly effective in treating dogs for the acute course of babesiosis and contributes to the normalization of hematological and biochemical parameters.

Keywords: DOGS, RED BLOOD CELLS, CRYOPRESERVATION, BABESIOSIS, TRANSFUSION, HEMATOLOGICAL AND BIOCHEMICAL PARAMETERS

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