Bìol. Tvarin, 2018, volume 20, issue 2, pp. 82–88

CHANGES IN THE NUMBER OF HEMOLYMPH IN BEES AFTER THE USE OF BIOSTIMULATOR “BIOCONTACT PLUS”

S. F. Tushak, T. O. Romanishina, Zh. V. Rybachuk, L. F. Lemeshynska
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Zhytomyr National Agroecological University,
39 Korolyova str., Zhytomyr 10025, Ukraine

The sources devoted to the bees’ immunity and its formation as well as the he studies of modern scientists on the changes in the quantitative and qualitative composition of hemolymph influenced by the treatment of known stimulants have been analyzed. The formation of cellular immunity has been studied, the changes in the hemolymph composition caused by natural and artificial factors have been analyzed. The effect of the drug “Biocontact Plus” as a stimulant for elderly bees has been analyzed and studied. The basic concentrations of the drug for use in beekeeping for prophylaxis and treatment of bees diseases have been determined. The main quantitative and morphological changes in the cellular composition of hemolymph caused the use of the drug with certain concentrations of “Biocontact Plus” have been studied.

The composition of hemolymph affected by the use of high concentratated drug has been analyzed. The toxicity of the drug in concentration 0.3 % was confirmed. The stimulating effect of the drug in concentrations 0.1 % and 0.15 % was noted. The dynamics of the increase of a number of precursor cells — proleucytes and a number of secretory cells — spherulocytes and encitoides with the given concentrations was detected, moreover the decrease of phagocyte cells — eosinophils and neutrophils with concentrations of 0.15 % and 0.1 % was recorded. Changes indicating the stimulating effect of the drug with concentrations 0.03 % and 0.01 % were not detected. Quantitative data describing cellular hemolymph composition with these concentrations differed from the control by less than 1 %. Certain morphological changes of hemocytes were described. With the use of the drug in the concentration, showing the toxic effect, the main morphological changes hemocytes were revealed. After 3–8 days of the experiment, the formation of pseudoresemlances on the opposite poles of the phagocytes was noted, and the cells became spindle-shaped. The displacement of nuclei in the spherulocytes caused by the feeding the stimulator with concentration 0.3 % was observed.

The stages of further research for a comprehensive study of the effect of the drug “Biocontact Plus” on bees of different generations in order to create an optimal scheme for the use of the drug for the prophylaxis and treatment of contagious diseases of honey bees are defined.

Keywords: HONEYBEES, HEMOLYMPH, PHAGOCYTE, BIOSTIMULATOR, BOICONTACT

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