Bìol. Tvarin, 2018, volume 20, issue 2, pp. 82–88


S. F. Tushak, T. O. Romanishina, Zh. V. Rybachuk, L. F. Lemeshynska
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Zhytomyr National Agroecological University,
39 Korolyova str., Zhytomyr 10025, Ukraine

The sources devoted to the bees’ immunity and its formation as well as the he studies of modern scientists on the changes in the quantitative and qualitative composition of hemolymph influenced by the treatment of known stimulants have been analyzed. The formation of cellular immunity has been studied, the changes in the hemolymph composition caused by natural and artificial factors have been analyzed. The effect of the drug “Biocontact Plus” as a stimulant for elderly bees has been analyzed and studied. The basic concentrations of the drug for use in beekeeping for prophylaxis and treatment of bees diseases have been determined. The main quantitative and morphological changes in the cellular composition of hemolymph caused the use of the drug with certain concentrations of “Biocontact Plus” have been studied.

The composition of hemolymph affected by the use of high concentratated drug has been analyzed. The toxicity of the drug in concentration 0.3 % was confirmed. The stimulating effect of the drug in concentrations 0.1 % and 0.15 % was noted. The dynamics of the increase of a number of precursor cells — proleucytes and a number of secretory cells — spherulocytes and encitoides with the given concentrations was detected, moreover the decrease of phagocyte cells — eosinophils and neutrophils with concentrations of 0.15 % and 0.1 % was recorded. Changes indicating the stimulating effect of the drug with concentrations 0.03 % and 0.01 % were not detected. Quantitative data describing cellular hemolymph composition with these concentrations differed from the control by less than 1 %. Certain morphological changes of hemocytes were described. With the use of the drug in the concentration, showing the toxic effect, the main morphological changes hemocytes were revealed. After 3–8 days of the experiment, the formation of pseudoresemlances on the opposite poles of the phagocytes was noted, and the cells became spindle-shaped. The displacement of nuclei in the spherulocytes caused by the feeding the stimulator with concentration 0.3 % was observed.

The stages of further research for a comprehensive study of the effect of the drug “Biocontact Plus” on bees of different generations in order to create an optimal scheme for the use of the drug for the prophylaxis and treatment of contagious diseases of honey bees are defined.


  1. Fedoruk R. S., Gavraniak A. R., Kovalchuk I. I. Factors of formation of immunity of honeybee bees. The Animal Biology, 2009, no. 1–2, pp. 83–90. (in Ukrainian)
  2. Galatiuk O. Y., Kisterna O.S. Scientific and practical recommendations. Study of hemolymph of honeybee bees (analysis and improvement of production of haemolymphatic smears). Kyiv-Sumy, 2015, 64 p.
  3. Gayfullina L. R., Saltykova E. S., Nikolaenko A. G. Cellular immunity of insects. Advances in modern biology, 2003, vol. 123, no. 2, pp. 195–206. (in Russian)
  4. Jones J. C. Regulation of Hemopoiesis. Ed. by A. .S Gordon. New York, Appleton-Century-Crofts, 1970, p. 7.
  5. Kysterna O. S., Garkava A. V., Musienko S. V., Musienko V. M. Estimation of hemolymph of honeybee bees with the use of biological stimulants under laboratory conditions. Visnyk SNAU, 2012, no. 7 (31), pp. 34–39. (in Ukrainian)
  6. Rudenko Ye. V., Nemkova S. M., Masliy I. G. Biological products stimulate the development of bee colonies. Pasika, 1996, no. 7, p. 26. (in Ukrainian)
  7. Rudenko Ye. V., Nemkova S. M., Masliy I. G. Influence of varorotic invasion on cellular composition of hemolymph and ways of its correlation. Visnyk SNAU, 2001, no. 6, pp. 100–104. (in Ukrainian)
  8. Şapcaliu A., Pavel C., Savu V., Matei M., Rădoi I. Biochemical and Cytological Investigations on Haemolymph of Apis Mellifera Carpathica Bee in Stressful Conditions. Bulletin UASVM Animal Science and Biotechnologies, 2010, no. 67 (1–2), pp. 313–320. Available at: http://journals.usamvcluj.ro/index.php/zootehnie/article/view/5317.
  9. Zapolsky O. V. Morphological and cytochemical analysis of cells of the hemolymoformula of the worker bee. Cytology, 1976, vol. 18, no. 8, pp. 959–963. (in Russian)
  10. Zyuman B. V. Factors and mechanisms of nonspecific resistance of honey bee (Apismellifera L.) Abstract of the dissertation. Doctor of Veterinary Sciences. Moscow, 1991, 32 p. (in Russian)
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