Bìol. Tvarin, 2018, volume 20, issue 2, pp. 51–57

PECULIARITIES OF THE GENETIC STRUCTURE OF PADDLEFISH (POLYODON SPATHULA) OF CHERNIGІV POPULATION

Kh. Kurta1, O. Malysheva1, V. Babenko2, V. Spyrydonov3
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1Ukrainian laboratory of quality and safety of agricultural products,
National University of Life and Environmental Sciences of Ukraine,
7 Mashynobudivnykiv str., Chabany village, Kyiv region, 08162, Ukraine, This email address is being protected from spambots. You need JavaScript enabled to view it.

2OJSV “Chernihiv Fish Industry”,
Zhavynka village, Chernigiv region, 15583, Ukraine, This email address is being protected from spambots. You need JavaScript enabled to view it.

3Institute of Veterinary Medicine NAAS of Ukraine,
30 Donetska str., Kyiv 03151, Ukraine

Paddlefish (Polyodon spathula) as one of representatives of sturgeon fish attracts a particular attention among cultivating species due to its high biological value, taste and dietary qualities of its meat. Investigation of the population-genetic structure of paddlefish is important for monitoring the effectiveness of breeding works in the artificial reproduction of this species.

The purpose of our work was to determine the peculiarities of the genetic structure of paddlefish of the Chernihiv population by microsatellite DNA markers.

In the studies the PCR method by the following microsatellite DNA markers Psp12, Psp21, Psp26, Psp28 was used. The amplification products were denatured with formamide (Sigma, USA) and separated by capillary electrophoresis on the genetic analyzer, ABI Prism 3130xl Genetic Analyzer (Applied Biosystems, USA).

As a result of genetic analysis, 22 allelic variants were identified in Chernigov paddlefish population. The Psp28 (8 allelic variants) was the most polymorphous among studied loci, the Psp21 locus (3 allelic variants) was the least polymorphic. The mean number of alleles per locus (Na) was 6.25. The observed heterozygosity (Ho) and expected heterozygosity (He) ranged from 0,457 (Psp21) to 0,943 (Psp28) and 0,550 (Psp21) to 0,711 (Psp28) with average values of 0,750 and 0,640, respectively. The polymorphic information content (PIC) ranged from 0.482 (locus Psp21) to 0.666 (locus Psp28), the mean PIC was 0.584. The mean rate of power of exclusion (PE) was PE=0.559 and ranged from 0.153 to 0.884 for Psp21 and Psp28 loci, respectively. Based on our population-genetic calculations, it was found that the paddlefish population is characterized by a high level of polymorphism by studied microsatellite DNA markers (Psp12, Psp21, Psp26, Psp26), and the genetic structure of the population has predominance of heterozygous genotypes over homozygous. These results can be recommended for fish breeding farms for practical application and implementation of molecular-genetic certification of the paddlefish’s broodstock.

Keywords: PADDLEFISH, MICROSATELLITE, DNA-MARKERS, GENOTYPING, POLYMORPHISM, GENETIC STRUCTURE

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