Bìol. Tvarin, 2018, volume 20, issue 2, pp. 30–42


N. P. Harasym, O. I. Bishko-Moskalyuk, A. M. Shumska, D. I. Sanahursky

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Lviv National University named after Ivan Franko,
4 Hrushevskoho str., Lviv 79005, Ukraine, This email address is being protected from spambots. You need JavaScript enabled to view it.

Influence of histamine and sodium hypochlorite (SH), as well as their simultaneous action on structural features of rat kidneys on the 1st, 7th, 14th day of the experiment and after the rehabilitation period (21st day) is investigated using light microscopy and morphometric analysis.

It has been established that the exogenous administration of histamine rats at a dose of 1 μg/kg results in a decrease in the area of the kidneys corpuscles, vascular glomeruli at the 7th day, whereas the higher dose of the nutrient amine leads to morphometric indices in both 1st and 7th day of the experiment. It has been found that this biogenic amine causes narrowing of the lumen of the proximal and distal convoluted tubules in the cortical kidney. Under these conditions, cells are poorly perceived as a color, optically opaque, indicating the presence of metabolic changes.

SH at a concentration of 5 mg/l causes an increase in the area, as well as a larger and smaller axis of the vascular lobe on the 7th day of the experiment, while this substance in higher concentrations leads to significant cellular impairment for 1 day of the experiment. SH causes the development of hydropic dystrophy, disturbances in the structure of the membranes, as evidenced by their fuzzy contouring, increased permeability of blood vessels and capillaries. Changes in the size of the vasculature glomerular indicate a violation of the filtration process in the kidneys.

Under the combined effect of SH and histamine there is a decrease in the area of the kidney corpuscle, an increase in the area of the vascular glomerule, damaged cell membranes, distal and proximal tubules, increased vascular permeability, development of hydropsic dystrophy. Probably the decrease in the area of the kidney cells is due to damage to the cells of the proximal and distal tubules, the increase of which leads to compression of the Shumlyansky-Bowman capsule. Under the action of histamine, hydrogen peroxide, ammonia is formed, which leads to a toxic kidney damage. The simultaneous introduction of histamine and SH into the body rats is also likely to produce halogen derivatives that can affect kidney cells. These changes are less pronounced with the simultaneous action of SH and histamine at a dose of 1 μg/kg.


  1. Bereznyakova A. I. Pathological physiology. Kharkiv, Golden Pages Publishing House, 2003, 424 p. (in Ukrainian)
  2. Berhyn E. B. Pharmacology of the kidneys and its physiological bases. Moscow, Medicine, 1979, 336 p. (in Russian)
  3. Bishko O. I., Holovchak N. P., Boyko M. Y., Sanagursky D. I. Free radical processes in rats tissues under influence of sodium hypochlorite and histamine. Biophysical visnyk, 2014, vol. 31 (1), pp. 14–26. (in Ukrainian)
  4. Bodnar Ya. Ya., Fayfur V. V. Pathological anatomy and pathological physiology of a person. Ternopil, Ukrmedkniga, 2000, 494 p. (in Ukrainian)
  5. Campbell W. B., Itskovitz H. D. Effect of histamine and antihistamines on renal hemodynamics and functions in the isolated perfused canine kidney. J. Pharmacol. Exp. Ther., 1976, vol. 198 (3), pp. 661–667.
  6. Chaychenko G. M., Tsibenko V. O., Sokur V. D. Physiology of man and animals. Kyiv, Higher school, 2003, 463 p. (in Ukrainian)
  7. Chekman I. S. Clinical pharmacology of antihistaminic drugs. Medicine of railway transport of Ukraine, 2002, no 2, pp. 58–61. (in Ukrainian)
  8. Dryzhak V. I., Babanli S. R., Dombrovich M. I., Zagurska N. O. Efficacy of laser, ultraviolet irradiation, indirect electrochemical oxidation of blood in detoxication therapy of oncologic patients. Oncology, 2002, vol. 4, no. 2, pp. 281–284. (in Ukrainian)
  9. Eliseeva V. G. Basics of general histology and histological technique. Moscow, State Publishing House of Medical Literature MEDGIZ, 1959, 214 p. (in Russian)
  10. Estrela C., Silva J. A., Gonçalves de Alencar A. H., Leles C. R., Decurcio D. A.. Efficacy of sodium hypochlorite and chlorhexidine against Enterococcus faecalis — a systematic review. J. Appl. Oral. Sci., 2008, vol. 16, no. 6, pp. 364–368https://doi.org/10.1590/S1678-77572008000600002
  11. Gurgen S. G., Erdogan D., Take-Kaplanoglu G. The effect of histamine on kidney by fasting in rats. Bratisl. Lek. Listy, 2013, vol. 114 (5), pp. 251–257. https://doi.org/10.4149/BLL_2013_052
  12. Ivashchenko V. V., Danilkov A. P., Golovanov S. A., Kirpatovsky V. I., Kudryavtsev Yu. V., Drozhzheva V. V. Sodium hypochlorite in the concentrating function of tubules. Experimental and clinical urology, 2010, no. 3. Available at: https://ecuro.ru/article/gipokhlorit-natriya-v-kontsentriruyushchei-funktsii-kanaltsev.
  13. Ivashchenko V. V., Kirpatovsky V. I., Kalabekov A. A., Kazachenko A. V., Grebenkin M. V., Golovanov S. A., Drozhzheva V. V. Changes in the electrolyte composition of urine under the influence of sodium hypochlorite. The possibility of reducing the risk of recurrence of nephrolithiasis. Experimental and clinical urology, 2017, no. 1. Available at: http://uroweb.ru/article/izmeneniya_elektrolitnogo_sostava_mochi_pod_deystviem_gipohlorita_natriya_vozmognost_umensheniya_riska_retsidiva_nefroli.
  1. Komarenko A., Terekhov A., Vorobyova A. Investigation of the role of H1-receptors in histamine portal rats reaction of rat liver vessels. B. Cmelnytsky National University of Cherkasy. Biological series, 2008, vol. 128, pp. 54–58. (in Ukrainian)
  2. Konyukhov A. L. Guide to using the ImageJ software for image processing. A tutorial. Tomsk, Department of TU, TUSUR, 2012, 105 p. (in Russian)
  3. Kovacova-Hanuskova E., Buday T., Gavliakova S., Plevkova J. Histamine, histamine intoxication and intolerance. Allergol. Immunopathol. (Madr.), 2015, vol. 43, no. 5, pp. 498–506. https://doi.org/10.1016/j.aller.2015.05.001
  4. Likhachev A. G. A multivolume guide to otolaryngology. Vol. 4. Moscow, Book on Demand, 1963, 552 p.
  5. Nikolishin A. K., Geranin S. I. Usage of antiseptics and haemostatic agents at one-visit extirpation treatment method of pulpitis. World of Medicine and Biology, 2011, no. 1, pp. 121–127. (in Ukrainian)
  6. Peck B. W, Workeneh B., Kadikoy H., Abdellatif A. Sodium hypochlorite-induced acute kidney injury. Saudi J. Kidney Dis. Transpl., 2014, vol. 25 (2), pp. 381–384. https://doi.org/10.4103/1319-2442.128553
  7. Petrov S. I. The use of sodium hypochlorite in clinical toxicology. Dr. Medical sci. Moscow, 2005, 197 p. (in Russian)
  8. Pogoretskaya Ya. O. Physiology of the excretory system. Methodical instructions for students of medical faculty. Lviv, Lviv National Medical University named after Danylo Halytsky, 2017, 40 p. (in Ukrainian)
  9. Salmanov S. A. Sodium hypochlorite in the treatment and prevention of kidney insufficiency of ischemic and infectious genesis. Candidate of Medical Sciences thesis, Scientific Research Institute of Urology of Russian Health Care, Moscow, 2005, 174 p. Available at: http://www.dissercat.com/content/gipokhlorit-natriya-v-lechenii-i-profilaktike-pochechnoi-nedostatochnosti-ishemicheskogo-i-i#ixzz58IvPIeSO. (in Russian)
  10. Shevchuk V. G. Physiology. Vinnytsya, The New Book, 2012, 448 p. (in Ukrainian)
  11. Shlopov V. G. Pathological anatomy. Vinnytsya, New book, 2004, 768 p. (in Ukrainian)
  12. Sklyarov O. Ya. Clinical biochemistry. Kyiv, Medicine, 2000, 432 p. (in Ukrainian)
  13. Stepanskiy D. A., Kremenchutskiy G. M., Koshevaya I. P., Toropin N. V., Toropin V. N. Research of antimicrobial properties of sodium hypochlorite solution and taurine. Biomedical and biosocial anthropology, 2014, no. 22, pp. 79–82. (in Russian)
  14. Vander A. Physiology of the kidneys. St. Petersburg, Pyter, 2000, 252 p. (in Russian)
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