Bìol. Tvarin, 2018, volume 20, issue 2, pp. 24–29


O. E. Galatiuk1, O. R. Kalnaus1, V. O. Volynets2, O. O. Kucheryavenko2

This email address is being protected from spambots. You need JavaScript enabled to view it., This email address is being protected from spambots. You need JavaScript enabled to view it.

1Zhytomyr National Agroecological University,
7 Staryy bulvar str., Zhytomyr 10008, Ukraine, This email address is being protected from spambots. You need JavaScript enabled to view it.

2Institute of Veterinary Medicine NAAS,
30 Donetska str., Kyiv 03151, Ukraine, This email address is being protected from spambots. You need JavaScript enabled to view it.

The article presents the results of research on the improvement of therapeutic and prophylactic measures for the latent course of leptospirosis in horses through the use of polyvalent inactivated vaccine. The vaccine was made in the laboratory of leptospirosis with the Museum of Microorganisms at the Institute of Veterinary Medicine National Academy of Agrarian Sciences of Ukraine.

In one of the equestrian farms with a latent perception of leptospirosis, 150 horses were immunized. To study the immunization effectiveness of this vaccine and the immunity formation, 19-year-old horses were selected: 4 stallions-sires aged 6 to 12 years, 5 mares aged 6–10 years, 5 sport horses aged 3–5 years and 5 young ones at the age of 1–2 years. The vaccine against leptospirosis of horses contained the following serogroups, serovars and leptospiros strains, respectively: 1) Grippotyphosa — grippotyphosa VGNKI-1; 2) Canicola canicola-VGNKI-3; 3) Icterohaemorrhagiae — icterohaemorrhagiae-VGNKI-2; 4) Australis-bratislava Yez bratislava. The vaccine was injected intramuscularly at a dose of 5 cm3. For research of the microagglutination reaction blood was taken before vaccination, after 1, 2, 4, 5, 6.5 months after vaccination.

At the 5th and 6th months after the application of the vaccine, in all the different age and sex groups of horses the titres of antibodies to the Australis, Hebdomadis, Sejrое serogroups increased, but we did not notice any clinical signs of the disease. That indicates a latent form of leptospirosis in the farm. In addition, the titres of antibodies increased as much as possible in the stallions-sires at the 5th month after vaccination, while the 6th month showed a downward trend. Meanwhile in mares, sport horses and young animals the antibody titers went up at the 5th and 6th months after vaccination.

The obtained data testify that the use of polyvalent vaccine against lethospirosis of horses (Equi variant) contributes to the formation of intense immunity in the latent period of leptospirosis of horses and it is advisable to use it in horse farms.


  1. Alekseyeva G. Yu. Leptospirosis of animals (diagnostics, etiological structure, risk prediction). Autoref. Of PhD thesis in vet. science. Kyiv, 2016, 22 p. (in Ukrainian)
  2. Galatyuk A. E, Antonyuk A. A, Kalnaus O. R. Formation of specific immunity against leptospirosis in horses using the Bovis vaccine. Scientists notes UO VGAVM, 2016, vol. 52, no. 3, pp. 26–30. (in Russian)
  3. Galatyuk O. E. Leptospirosis Prevention and treatment of infectious diseases in horses. Zhytomyr, Publishing House “Ruta”, 2009, 399 p. (in Ukrainian)
  4. Instruction on laboratory diagnosis of leptospirosis: registered. Jan. 11 1997, no. 15–14/2. Kyiv, 1996, 28 p. (in Ukrainian)
  5. Leptospirosis. Leptospirosis in Cattle, Pigs, Sheep, Horses and Humans. Available at: http://www.livinglegends.org.au/horse-health/horse-diseases/leptospirosis-in-cattle-pigs-sheep-horses-and-humans.
  6. Mandingra M. C. Epizootic and epidemiological aspects of leptospirosis in Ukraine. Kyiv, 2015, 46 p. (in Russian)
  7. Mason C. A. Lepto EQ Innovator. Available at: http://claramason.com/new-lept.pml.
  8. Volynets V. O., Kucheryavenko O. O., Ukhovsky V. V., Kulikova V. V. Epizootic situation regarding leptospirosis of horses in Ukraine. Scientific Herald LNAVM named by Gzhytsky, 2013, vol. 15, no. 3 (57), pp. 49–53. (in Ukrainian)
  9. Veterinary medicine. Methods of laboratory diagnosis of leptospirosis: DSTU 6078: 2009, National Standart of Ukraine. Kyiv, 2009, 26 p. (in Ukrainian)
  10. Volynets V. O. Tests of a polyvalent inactivated vaccine against leptospirosis in horses. Animal husbandry of Ukraine, 2014, vol. 5, pp. 43–46. (in Ukrainian)
© 2016 Institute of Animal Biology