Bìol. Tvarin, 2018, volume 20, issue 1, pp. 9–15
M. M. Babkina1, O. V. Vasylchenko2, O. M. Deriabin3, A. A. Tarasov4A. M. Golovko3, L. G. Palchykovska2
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1Institute of Animal Biology NAAS,
38 V. Stus str., Lviv 79034, Ukraine
2Institute of Molecular Biology and Genetics NAS of Ukraine,
150 Akademika Zabolotnoho str., Kyiv 03680, Ukraine
3State Scientific Control Institute of Biotechnology and States of Microorganisms,
30 Donetska str., Kyiv 03151, Ukraine
4Institute of Veterinary Medicine NAAS,
30 Donetska str., Kyiv 03151, Ukraine
The results of the study of antibacterial activity and determination of minimum inhibitory concentration substituted acridones class by microdilution method against a Staphylococcus aureus are described in the article. The antibacterial activity of the substituted acridones class was investigated by disk diffusion method. An antibacterial activity of substituted acridones class to museum strain to Staphylococcus aureus was established. The presence of the susceptibility of Staphylococcus aureus to the tested compounds has been revealed.
In this research we used the substituted acridones class compounds. We chose fourteen the most perspective substances with some difference due to the position of radical groups and it composition recording the results of the research.
As the result of investigation the minimal inhibitory concentration and growth of inhibition zones of substituted acridones class substances was identified. The minimal inhibitory concentration was detected at range from 0.41±0.004 mg/cm3 to 0.0041±0.00005 mg/cm3 and growth zone diameter was from 8.2±0.4 mm to 24.1±0.31 mm.
The most active compounds tested of substance regards to Staphylococcus aureus were ODI-90 (2,5-difluoro-9-oxo-N-pyridin-4-yl-9,10-dihydroacridine-4-carboxamide) with a minimum inhibitory concentration 0.0041±0.00005 mg/cm3 and the diameter of growth inhibition zone 24.1±0,31mm. Compounds ODI-76, ODI-77, ODI-80, ODI-85 had the minimum inhibitory concentration 0.041 mg/cm3 and the diameter of growth inhibition zone from 20.8±0.34 mm to 21.2±0.4 mm. The compounds ODI-64, ODI-65, ODI-68, ODI-71, ODI-72, ODI-73, ODI-84, ODI-88, ODI-89 which differ by their nature and position of the radicals demonstrated low level of antibacterial activity with a minimal inhibitor concentration. The diameter of the zones of growth inhibition for these compounds was from 8.2±0.4 mm to 14.0±0.36 mm.
The compounds ODI-61, ODI-62, ODI-63, ODI-66, ODI-67, ODI-69, ODI-70, ODI-74, ODI-75, ODI-78, ODI-79, ODI-81, ODI-82, ODI-83, ODI-86, ODI-87 did not demonstrate any activity against Staphylococcus aureus.


  1. Avilov S. Golden and deadly. Around the world, 2007, no. 3. Available at: http://www.vokrugsveta.ru/telegraph/pulse/279.
  2. Chambers H. F. The changing epidiology of Staphylococcus aureus. Emerging Infectious Diseases journal, 2001, 2, pp. 178–182.
  3. Isaev S. G., Yaremenko V. D., Suleiman M. M., Mamedova D. O., Kabakova N. D., Briznitztskii O. A., Shevelova N. Yu. Synthesis, physical, chemical and biological properties of 5-nitroderivatives acridine. Pharmacy of Ukraine. View in the future. Materials of VII national congress of pharmacists, Kharkiv, NPhaU, 2010, is. 1, p. 600. (in Ukrainian)
  4. Isaev S. G., Blizniuk O. A., Kruglenko N. V., Sumska O. P., Kabakova N. D., Palii G. K., Kryzhanovska A. V. Antrinilates of 9-aminoacridyne: synthesis, structure and antifungal, antimicrobial activity, their using as a coloring of textile products. Pharmacy of Ukraine. View in the future. Materials of VII national congress of pharmacists, Kharkiv, NPhaU, 2010, is. 1, p. 600. (in Ukrainian)
  5. Kluytmans J., van Belkum A., Verbrugh H. Nasal carriage of Staphylococcus aureus: epidemiology, underlying mechanisms, and associated risks. Clinical Microbiology Reviews, 1997, vol. 10 (3), pp. 505–520.
  6. Lorian V. Antibiotics in laboratory medicine. Baltimore, Williams and Wilkins, 1996, 642 p.
  7. National Committee for Clinical Laboratory Standards. Performance Standards for Antimicrobial Disk Susceptibility Tests. 4th Edition: Approved Standard M2-A4. NCCLS, Villanova, PA, 1990.
  8. Weidemann B. Evaluation of data from susceptibility testing. International journal of antimicrobial agent, 1998, 10, pp. 218–219.
  9. Witte W. Medical Consequences of Antibiotic Use in Agricultural Science. Science, 1998, vol. 13, pp. 996–997. https://doi.org/10.1126/science.279.5353.996
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