Bìol. Tvarin, 2017, Volume 19, Issue 1, pp. 125–134

MICROELEMENTS CONTENT IN TISSUES OF FEMALE F0 RATS AND F1 MALES AT THE WATERING OF NANO AND CHEMICALLY SYNTHESIZED GERMANIUM CITRATE

M. I. Khrabko1, R. S. Fedoruk1, M. I. Khrabko2, E. E. Martsinko3, H. H. Denys1

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1Institute of Animal Biology NAAS,
38 Vasyl Stus str., Lviv, 79034, Ukraine

2Vyshnyanskyy College Lviv National Agrarian University,
v. Vyshnyа, Lviv region, 81540, Ukraine

3I. I. Mechnikov Odesa National University,
2 Dvoryanska str., Odesa 65082, Ukraine

The results of the impact of long watering different amounts of citrate germanium nanotechnology and obtained by chemical synthesis, on the content of microelements in tissues of female and male mothers to their offspring are presented in the paper. A credible intergroup gender and age differences content of copper, cobalt, manganese, iron and zinc in tissues of liver, kidneys, lungs and muscles have been established.

In the tissues of female rats in the 2nd experimental group after watering 10 mg Ge, the content of microelements is the highest, as it is indicated by the significant increase of Cu in all organs, Co, Mn and Zn —in kidneys, lungs and muscles of female rats. Increasing of Ge concentrations in the water causes the reduction of Co, Mn and Zn in these organs, which is confirmed by watering chemically synthesized germanium. Increasing doses of citrate Ge 20 and 200 mg influenced the reduction of Co and Mn in all tissues of males in 3rd and 4th groups. Some differences between the yields of microelements in tissues of liver, kidneys, lungs and muscles of males and females in 2nd group receiving 10 mg Ge are typical. For the 3rd and the 4th groups the content of most of the elements kept their tendency in both females and males of these groups, except lower Zn content in kidneys, Fe — in muscles in males of 3rd group, but higher levels of Mn in this tissue and Fe — in lungs and muscles in 4th group and lower Cu in the muscles of these males. Watering chemically synthesized citrate Ge in amount 200 and 2000 mg of Ge led to unidirectional changes in the tissues of the internal organs and muscles of the likely increase in the level of Co and Mn in the liver, lungs and muscles, Cu — in lungs and muscles, Mn — in kidneys and muscles in female rats of 5th group and Zn — in muscles in female rats of the 5th and the 6th,in kidneys in the 6th groups against the backdrop of lower (P<0,01) Cu content in the liver in the 5th, Co and Mn in liver, lungs and muscles, Cu and Co in kidneys in females of the 6th group. The content of microelements in tissues of males of 6th group maintained tendency of significant difference in their mothers compared with control group for most of the tissues and elements, including: liver, besides Zn, kidneys and lungs, in addition to Fe and Zn, and muscles except Cu.

Uneven effects of different doses of citrate Ge, obtained by nanotechnology and chemical synthesis, on the level of the individual elements in the tissues of females and males from their offspring, confirmed the absolute content of trace elements in terms of weight investigated organs for which the same tendency of intergroup differences in most definitions was kept for both mothers F0 and their offspring F1.

Keywords: RATS, GERMANIUM CITRATE, TISSUES, MICROELEMENTS

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