Bìol. Tvarin, 2017, Volume 19, Issue 1, pp. 111118


T. Suprovich1, N. Mokhnachova2

kokas2008@ukr.net, nm82@i.ua

1State Agrarian and Engineering University in Podillya,
13 T. Shevchenko str., Kamyanets-Podilsky
32316, Ukraine

2The institute of Animals Breeding and Genetics named after M. V. Zubets NAAS,
1 Pogrebnyak str., Chubynske village, Boryspil district, Kyiv region, 08321
, Ukraine

For recent years the disappearance of many species of living beings, including the populations of farm animals is observed. Most of them are local (native) species. Humanity is gradually losing unique gene pool of domestic animals that have a number of economically useful traits. Therefore, the problem of preserving one of the oldest local species the grey Ukrainian breed is particularly relevant. It is important to study the issue of grey cattle complex of genetic factors that cause the phenotype of unique features, especially the ability to use this breed in the selection of securities for the economically useful traits.

Two populations of grey Ukrainian cattle breeds for QTL-markers that cause milk production and quality of meat have been investigated. Blood samples at 84 cows from the farm Markeyevo” (herd no.1) and 40 cattle farms Polyvanivka” (herd no. 2) have been used. The spectrum of alleles of genes of growth hormone (GH), beta-lactoglobulin (βLG), kappa-casein (CSN3), thyroglobulin (TG5) and сalpayn (CAPN) was studied using PCR-RFLP.

The study of genetic polymorphisms of genes of two populations of Ukrainian grey breed shows that gene of kappa-casein has prevalence of genotype AB (0.476), while the opposite distribution of frequencies has alleles A and B. For alleles, gene thyroglobulin is observed a similar distribution of C and T. The prevalence of genotype CT (0.571) in animals of herd no. 1 and excess of homozygotes in cows of herd no. 2 is found. For gene calpain in the herd no. 1 all the animals were carriers of genotype homozygous for the allele G (1.0). However, in the allelic spectrum of animals of herd no. 2 allele A (0.663) and its homozygous genotype dominated.

For animals from the farm “Markeyevo genetic spectrum alleles of genes βLG and GH is defined. For gene beta-lactoglobulin a significant prevalence of allele B is found, which appears 3 times more than the A allele that causes very high frequency manifestation of the genotype BB (0.512). For growth hormone gene we revealed a significant prevalence of genotype LL (0.964) and, accordingly, allele L (0.982), and the lack of genotype VV.


1. Babenko H. I. Relations of somatotropin hormone in ukrainian black and white dairy cattle breed and its association with productivity traits. Animal Breeding and Genetics, 2015, no. 49, pp. 148–153. (in Ukrainian)
2. Balackij V. N. Genetic polymorphism of soma-totropina Association and its alleles with quantitative traits of animals. Agricultural biology, 1998, no. 4, pp. 43–53. (in Ukrainian)
3. Bovenhuis H., Van Arendonk J. A. M., Korver S. Associations between milk protein polymorphisms and milk production traits. J. Dairy Sci., 1992, no. 75, pp. 2549–2559. https://doi.org/10.3168/jds.S0022-0302(92)78017-5
4. Dybus A., Grzesiak W., Kamieniecki H., Szatkowska I., Sobek Z., Blaszczyk P., Czerniawska-Piatkowska E., Zych S., Muszynska M. Association of genetic variants of bovine prolactin with milk production traits of Black-and-White and Jersey cattle. Arch. Tierz., Dummerstorf, 2005, vol. 48, no. 2, pp. 149–156.
5. Eggena Fries R. Die Untersuchung von Kaseingenenmittels DNA-Analyse. ETH Lan-dwirtschaft Schweb Band, 1992, pp. 231–235.
6. Grodziker T. Physical mapping of temperature-sensitive mutations of adenoviruses. Cold Spring Harbor Symp. Quaint. Biol., 1974, vol. 39, pp. 439–446. https://doi.org/10.1101/SQB.1974.039.01.056
7. Kharytonova S. N., Hlazko T. T., Kuznetsova O. V. State of the World’s Animal Genetic Resources for Food and Agriculture. Moscow, Rome, FAO, 2010, 512 p. (in Russian)
8. Khatamy S. R., Lazebnyy O. E., Maksymenko V. F., Sulymova H. E. DNA gene polymorphism of growth hormone and prolactin in the Yaroslavl and black-motley cattle in connection with milk production. Genetics, 2005, vol. 42, no. 2, pp. 229–236. (in Russian)
9. Kopylova K. V., Kopylov K. V., Tarasiuk S. I., Metlitska O. I. Polymorphisms of genes associated with economically useful traits in cattle. Journal of Agricultural Science, 2006, no. 10, pp. 52–58. (in Ukrainian)
10. Kurak O. P., Gandga A. I., Gurina N. V., Letkevich L. L., Simonenko V. P., Kovalchuk M. A., Kirillova I. V. Monitoring cattle Belarusian black-motley breed on the loci of economically significant traits. Animal Breeding and Genetics, 2014, no. 48, pp. 194–202. (in Ukrainian)
11. Perchun A. V., Lazebnaya I. V., Belokurov S. G., Ruzina M. N., Sulimova G. E. Polymorphism of genes CSN3, bPRL I bGHu cows of Kostroma breed in connection with the performance of milk production. Basic research, 2012, no. 11–2, pp. 304–308. (in Russian)
12. Sheremeta V. I. Genetic diversity of species in Ukraine FAO report. Preservation of the gene pool of animals: Materials creative discussions. Kyiv, Agricultural science, 2007, pp. 90–96. (in Ukrainian)
13. Stolpovskaya O. V., Stolpovskiy Yu. A., Godovanets L. V. A comparative study of polymorphism of proteins Ukrainian gray breed. Cytology and Genetics, Kyiv, 1992, no. 26 (5), pp. 11–17. (in Ukrainian)
14. Suprovich T. M., Kopylov K. V. Determination of DNA markers in susceptible and resistant to mastitis cows Ukrainian black and white dairy cattle. Animal Breeding and Genetics, 2014, no. 48, pp. 214–223. (in Ukrainian)
15. Zinoveva N. A. Sample preparation, DNA extraction and optimization PCR analysis. Methods of research in biotechnology livestock: School-Workshop, VIJ, 2002, pp. 33–45. (in Russian)
16. Zinoveva N. A., Kostunica O. V., Gladyr E. A. DNA diagnostics polymorphisms gene-proteins of milk bovine. Methods of research in biotechnology livestock. Moscow, 2004, pp. 7–22. (in Russian)
17. Zubets M. V., Burkat V. P., Huzyev I. V. Solving the problem of maintaining genetic diversity in livestock of Ukraine. Journal of Agricultural Science, 2008, no. 12, pp. 7–10. (in Ukrainian)

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