Bìol. Tvarin, 2017, Volume 19, Issue 1, pp. 93–99


Y. F. Rivis1, N. E. Yanovych2

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1Institute of Agriculture of Carpathian Region NAAS,
5 Grushevskogo str., Obroshino
, Lviv Oblast, 81115, Ukraine

2Lviv National University of Veterinary Medicine and Biotechnologies named after S.Z.Gzhytsky,
50 Pekarska str., Lviv 79010, Ukraine

Copper and Zinc are essential for fishes elements with wide spectrum of biological activity; in particular, they are involved in regulation of fatty acids metabolism. Content and ratio of fatty acids in fish meat is directly influenced with its nutritive and biological value; besides, fatty acids composition of cells membranes determines their penetration for xenobiotics, including heavy metals. Thus, investigation of the influence of mentioned elements on the fatty acids metabolism in tissues of fishes has both theoretical and practical importance.

The experiment was conducted on three groups (10 fishes in each group) of two year old common carp (Cyprinus carpio L.). Carps were kept for 21 days without feeding in aquariums with different concentration of Copper and Zinc in the water 0.3 and 4.2 mg/l correspondingly for control group, one maximum permitted level (1 MPL) for the 1st experimental group and 2 MPL for the 2nd experimental group. At the end of the experiment carps of each group were weighted, and samples of the gills were taken after slaughter for laboratory research. Concentration of copper and zinc in the gills was determined by spectrometric method, and concentration of non-etherified fatty acids was measured by gas chromatographic method.

Presented results shows that at 1 MPL of Zinc and Copper in the water, their concentration in carp gills increases by 9.31 % (P<0.020.05) and 29.54 % respectively in comparison to the fishes of the control group. At 2 MPL of Zinc and Copper in the water, Zinc increases in carp gills by 16.49 % (P<0.01), and Copper increases by 104.54 % (P<0.001) in comparison to the control group. Increasing of Copper and Zinc concentration in carp gills is accompanied by changes of the concentrations of non-etherified fatty acids in them. In particular, at 1 MPL of Copper and Zinc in the water, the increasing of total content of high metabolically active non-etherified fatty acids in carp gills was observed. At the same time, at 2 MPL of Copper and Zinc in the water, total content of high metabolically active non-etherified fatty acids in the gills of carps was decreased. Changes of Copper, Zinc and non-etherified fatty acids concentrations in the gills of carps were accompanied by changes of their live weight in the end of the experiment. Carps of the control group lost 3.90 % of live weight, and carps of the 1st and the 2nd experimental groups — 4.91 and 9.75 % (P<0.02–0.05) respectively.


1. Appendix A of the European convention for the protection of vertebrate animals used for experimental and other scientific purposes (Ets no. 123). Guidelines for accommodation and care of animals (Article 5 of the Convention) approved by the multilateral consultation. Strasbourg, 15 June 2006, 109 p.
2. Clearwater S. J., Farag A. M., Meyer J. S. Bioavailability and toxicity of diet borne Copper and Zinc to fish. Comp. Biochem. Physiol. C. Toxicol. Pharmacol., 2002, vol. 132, no. 3, pp. 269–313. https://doi.org/10.1016/S1532-0456(02)00078-9
3. Dhanapakiam P. Toxic effects of copper and zinc mixtures on some haematological and biochemical parameters in common carp, Cyprinus carpio (linn). J. Environ. Biol., 2001, vol. 22, pp. 105–111.
4. Evtushenko N. Y., Malugeva T. D. Intensity of protein synthesis in carp liver at keeping in water with different zinc concentration. Theses of report of Second Union Conference on the use of warm waters of TPS and APS for fisheries purposes, Moscow, 1980, pp. 26–27. (in Russian)
5. Hongxia J., Hongmei Y., Xianghui K., Shuping W. Huiyun G. Changes of superoxide dismutase and catalase activities in crucian carp (Carassius auratus) exposed to copper and recovery response. Life Sci. J., 2013, 10 (1), pp. 3281–3288.
6. Hrytsyniak I. I., Smolyaninov K. B., Yanovych V. G. Lipids metabolism in fish. Lviv, Triada plus, 2010, 336 p. (in Ukrainian)
7. Hrytsyniak I. I., Yanovych D. O., Schvets T. M. Ecotoxicology of Salmonids. Kyiv, Ltd. “DIA”, 2015, 472 p. (in Ukrainian)
8. Huang Y. S., Cunnane S. C., Horrobin D. F., Davignon J. Most biological effects of Zinc deficiency corrected by gamma-linolenic acid (18:3 omega 6) but not by linoleic acid (18:2 omega 6). Atherosclerosis, 1982, 41, pp. 193–207. https://doi.org/10.1016/0021-9150(82)90185-X
9. Kurant V. Z., Brodin S. V., Syniuk Y. V. The influence of heavy metals on glycine metabolism in tissues of carp (Cyprinus carpio L.). Materials of Metals and Cell Symposium Canterbury, University of Kent at Canterbury, 2001, 45 p.
10. Kurant V. Z., Chomenchuk V. O., Bujak V. Y. Ways of penetration and content of heavy metals in fish body. A review. Scientific Notes of Ternopil National Pedagogical University named after Volodymyr Hnatiuk, Series Biology, 2011, vol. 2 (47), pp. 263–269. (in Ukrainian)
11. Manyora G. B., Grubinko V. V. Dynamics of lipid composition of fish brain at intoxication of heavy metals ions. Hudrobiological J., 2004, vol. 40, no. 5, pp. 49–56. (in Russian)
12. Price W. J. Analytical Atomic Absorption Spectrometry. Heyden and Son Ltd., London, 1972, 239 p.
13. Rajamanickam V., Muthuswamy N. Effect of heavy metals induced toxicity on metabolic biomarkers in common carp (Cyprinus carpio L.). Mj. Int. J. Sci. Tech., 2008, 12 (01), pp. 192–200.
14. Reed S., Xia Qin, Ran-Ressler R., Brenna J.-T., Glahn R. P., Tako E. Dietary Zinc deficiency affects blood linoleic acid: dihomo-γ-linolenic acid (LA:DGLA) ratio; a sensitive physiological marker of Zinc status in vivo (Gallus gallus). Nutrients, 2014, 6 (3), pp. 1164–1180. https://doi.org/10.3390/nu6031164
15. Rivis J. F., Fedoruk R. S. Quantitative and qualitative chromatographical methods of some lipids and fatty acids determination in biological material. Lviv, Spolom, 2010, 110 p. (in Ukrainian)
16. Sandor Z., Csengeri I., Oncsik M. B., Alexis M. N., Zubcova E. Trace metal levels in freshwater fish, sediment and water. Environ. Sci. Pollut. Res. Int., 2001, 8 (4), pp. 265–268. https://doi.org/10.1007/BF02987404
17. Standards of water quality for facilities for fisheries purposes, including standards of maximum permitted levels of harmful substances in water of facilities for fisheries purposes. Moscow, VNIRO, 2011, 257 p. (in Russian)
18. Štrbac S., Kašanin-Grubin M., Jovančićević B., Simonović P. Bioaccumulation of Heavy Metals and Microelements in Silver Bream (Brama brama L.), Northern Pike (Esox lucius L.), Sterlet (Acipenser ruthenus L.), and Common Carp (Cyprinus carpio L.) From Tisza River, Serbia. J. Toxicol. Environ. Health A., 2015, 78 (11), pp. 663–665. https://doi.org/10.1080/15287394.2015.1023406
19. Wahle K. W. J., Davies N. T. Effect of dietary Copper deficiency in the rat on fatty acid composition of adipose tissue and desaturase activity of liver microsomes. British Journal of Nutrition, 1975, 34, pp. 105–112. https://doi.org/10.1017/S000711457500013X
20. Watanabe T., Kiron V., Satoh H. Trace minerals in fish nutrition. Aquaculture, 1997, 151, pp. 185–207. https://doi.org/10.1016/S0044-8486(96)01503-7
21. Yanovych N. E. Fatty acids composition of carp skeletal muscles under influence of different copper and zinc concentration in water. Fisheries Science of Ukraine, 2013, vol. 2, pp. 70–75.
22. Yanovych N. E. Influence of different copper and zinc concentration in water on fatty acids composition of carp liver. Fisheries Science of Ukraine, 2013, vol. 1, pp. 50–57.
23. Yanovych N. E., Yanovych D. O. Role of trace elements in pond fishes vital functions. Science herald of LNUVM and BT named after S. Z. Gzhytsky, 2014, vol. 16, no. 2, pp. 345–372. (in Ukrainian)

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