Bìol. Tvarin, 2017, Volume 19, Issue 1, pp. 93–99

PECULIARITIES OF NON-ETHERIFIED FATTY ACIDS CONTENT IN CARP GILLS AT DIFFERENT CONCENTRATION OF COPPER AND ZINC IN THE WATER

Y. F. Rivis1, N. E. Yanovych2

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1Institute of Agriculture of Carpathian Region NAAS,
5 Grushevskogo str., Obroshino
, Lviv Oblast, 81115, Ukraine

2Lviv National University of Veterinary Medicine and Biotechnologies named after S.Z.Gzhytsky,
50 Pekarska str., Lviv 79010, Ukraine

Copper and Zinc are essential for fishes elements with wide spectrum of biological activity; in particular, they are involved in regulation of fatty acids metabolism. Content and ratio of fatty acids in fish meat is directly influenced with its nutritive and biological value; besides, fatty acids composition of cells membranes determines their penetration for xenobiotics, including heavy metals. Thus, investigation of the influence of mentioned elements on the fatty acids metabolism in tissues of fishes has both theoretical and practical importance.

The experiment was conducted on three groups (10 fishes in each group) of two year old common carp (Cyprinus carpio L.). Carps were kept for 21 days without feeding in aquariums with different concentration of Copper and Zinc in the water 0.3 and 4.2 mg/l correspondingly for control group, one maximum permitted level (1 MPL) for the 1st experimental group and 2 MPL for the 2nd experimental group. At the end of the experiment carps of each group were weighted, and samples of the gills were taken after slaughter for laboratory research. Concentration of copper and zinc in the gills was determined by spectrometric method, and concentration of non-etherified fatty acids was measured by gas chromatographic method.

Presented results shows that at 1 MPL of Zinc and Copper in the water, their concentration in carp gills increases by 9.31 % (P<0.020.05) and 29.54 % respectively in comparison to the fishes of the control group. At 2 MPL of Zinc and Copper in the water, Zinc increases in carp gills by 16.49 % (P<0.01), and Copper increases by 104.54 % (P<0.001) in comparison to the control group. Increasing of Copper and Zinc concentration in carp gills is accompanied by changes of the concentrations of non-etherified fatty acids in them. In particular, at 1 MPL of Copper and Zinc in the water, the increasing of total content of high metabolically active non-etherified fatty acids in carp gills was observed. At the same time, at 2 MPL of Copper and Zinc in the water, total content of high metabolically active non-etherified fatty acids in the gills of carps was decreased. Changes of Copper, Zinc and non-etherified fatty acids concentrations in the gills of carps were accompanied by changes of their live weight in the end of the experiment. Carps of the control group lost 3.90 % of live weight, and carps of the 1st and the 2nd experimental groups — 4.91 and 9.75 % (P<0.02–0.05) respectively.

Keywords: NON-ETHERIFIED FATTY ACIDS, ZINC, COPPER, GILLS, CARP, LIVE WEIGHT

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