Bìol. Tvarin, 2017, Volume 19, Issue 1, pp. 65–72                    http://dx.doi.org/10.15407/animbiol19.01.065

BIOCHEMICAL MECHANISMS OF STRESS DEVELOPMENT IN RATS OF DIFFERENT AGE GROUPS UNDER THE INFLUENCE OF SODIUM NITRITE WITH THE TOBACCO INTOXICATION BACKGROUND

P. H. Lykhatskyi, L. S. Fira, L.E.Grytcishin

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Horbachevsky Ternopol State Medical University,
1
 Voli sq., Ternopil 46001, Ukraine

The article presents the results of the research of oxidative processes in rats of different age groups after the influence of sodium nitrite with the tobacco smoke intoxication during 45 days in the background.

The model of dependence on chronic tobacco smoke was created with the use of hermetic chamber with capacity 30 l which allowed fuming the animals in free behavior.

Rats were divided into 3 groups: young, mature and old ones. In each of these groups there was untreated control. For the study the blood, blood serum, liver, lungs, kidney and myocardium of animals was taken. The content of reactive oxygen species (ROS) in neutrophils, the content of TBA-active products in all target organs and the content of methemoglobin in the blood were determined.

The studies have shown that the simultaneous entry of mentioned toxicants into the organisms causes a significant increase in the content of reactive oxygen species (P≤0.05) in blood neutrophils in animal of all ages. With the backdrop of increased AFC content we marked activation of lipid peroxidation, which is indicated by the increased content of TBA-active products in all organs of rats after affection. The most pronounced activation of the processes of lipid peroxidation was observed in the myocardium of young animals. At the same time we have pointed out the activation of process of methemoglobin synthesis in the affected animals.

The results give reason to believe that young rats are the most sensitive at the simultaneous influence of sodium nitrite and tobacco smoke.

Keywords: BIOCHEMISTRY, SMOKE, SODIUM NITRITE, RATS, REACTIVE OXYGEN SPECIES, METHEMOGLOBIN, LIPID PEROXIDATION, TBA-ACTIVE PRODUCTS

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