UDC 636.2:618.591.11                                                  http://dx.doi.org/10.15407/animbiol18.04.086


G. V. Sobko


Institute of animal biology NAAS,
38 Vasyl Stus str., Lviv 79034, Ukraine

The results of experimental research of the influence of drug “Antymast” made on the basis of natural bee raw on indexes of T- and B- cells link of immunity in the blood of cows with subclinical mastitis are presented in the article.

The study was conducted in the spring period on two groups of black and white dairy cows (2nd3rd lactation) with 57 animals in a group. The cows were previously diagnosed by rapid method of counting somatic cells for subclinical forms of mastitis: control group contained clinically healthy animals and research group consisted of cows with signs of subclinical mastitis. Cows in research groups were administered one injection of tube (13ml) of the drug “Antymast” in the affected udder quarters three times with intervals of 24 hours and prophylactically a half therapeutic dose in healthy udder quarters. Blood for immunological studies was taken on the 1st day (before drug administration), on the 3rd and 9th day after its application.

It has been stated that in cows suffering from subclinical form of mastitis cellular link of immune system increased (P<0.05–0.01) the absolute number of leukocytes and decreased (P<0.05–0.01) the relative amount functional activity of T-lymphocytes and their subpopulations by regulatory receptor system of redistribution of immune cells.

Introduction of research drug “Antymast” helped to reduce the number of leukocytes in the blood. On the 9th day after the injection number of leukocytes in the blood decreased (P<0.05) and was at the same level as in animals of control group. In the blood of cows of research group at the 3rd day after the injection the increasing of the total number of T- and B- lymphocytes compared to the values of this index in clinically healthy animals was observed. The total number of T-lymphocytes (active and theophylline-resistant) and B-lymphocytes in the blood of cows of research group at the 9th day after the injection was higher (P<0.05–0.01) than in the control group. Thus, the number of Th-RUL RUL-EAS and low density of receptors in the blood of cows of experimental group was greater (P<0.01), and “zero” functionally undifferentiated cells was lower (P<0.01) compared to the control.


1. Baker C. E., Pradhan A. L. Pakstis. Synthesis and antibacterial properties of silver nanoparticles. J. of Nanoscience and Nanotechnology, 2005, vol. 2, no. 2, p. 244–247. https://doi.org/10.1166/jnn.2005.034

2. Balym Y. P., Novikov V. B. Distribution of subclinical (latent) mastitis in cows and its impact on the sanitary quality of milk. Veterinary Medicine: Int. temat. Science. Coll. 2000, no. 78, p. 17–22. (in Russian)

3. Basella R. P. Staphylococcus aureus capsule and slime as virulence factor in ruminant mastitis. Arevian. Veter. Microbiol., 1994, vol. 39, no. 3/4, pp. 195–204. https://doi.org/10.1016/0378-1135(94)90157-0

4. Brulyn A. P., Boyko A. B. Program on struggle with venerable and Improvement of milk quality. Veterinary Medicine, 2006, no. 5, pp. 9–12. (in Russian)

5. Devriese A. Z. Asimple identification scheme for coagulase negative Staph. from bovine mastitis. Res in veter. Sc., 1994, v. 57, no. 2, pp. 240–244.

6. Golovko A. M., Vechmotov V. B., Huzhvynska S. O. Diagnosis and treatment of cows suffering from subclinical mastitis. Problems of Zooengineering and Vet. Medicine, 2001, no. 8, pp. 237–239. (in Russian)

7. Kalorey D. R., Kolte R. M., Dhoot V. M., Dakshinkar N. P., Harte S. D. Non-antibiotic treatment for subclinical mastitis in cows. Indian Journal of Veterinary Medicine, 1993, vol. 1, no. 3, pp. 284–286.

8. Kosenko M. V., Serhiyechko O. I., Kovalchuk L. M. Changes in the cellular composition of milk during subclinical mastitis in cows. Bulletin of Lviv. State. Acad. Vet. Medicine, 2002, vol. 4, no. 5, pp. 247–248. (in Russian)

9. Owens W. E., Nsckerson S. C., Doddie R. L. Watts Combination therapy increases cure rates for bovine mastitis. Louisiana Agriculture, 1989, vol. 20, no. 2, pp. 6–7.

10. Parykov V. A., Klimov N. T., Romanenko A. I. Mastitis in cows. Veterinary Medicine, 2000, no. 11, pp. 34–37. (in Russian)

11. Ratnakumar A. V. Hamza P. A., Choudhuri P. C. Treatment of subclinical mastitis in early lactation. Indian Veterinary Journal, 1996, vol. 9, no. 14, pp. 970–972.

12. Rubtsov V. I. Prevention and Treatment of mastitis in cows. Veterinary Medicine, 2006, no. 9, pp. 32–35. (in Russian)

13. Rumar A. V. Hamza P. A., Choudhuri P. C. Economic implications of treating subclinical mastitis. Indian Journal of Dairy Science, 1996, vol. 7, no, 8, pp. 459–641.

14. Saxena R. K., Dutta G. N., Boragohain J. T. Drug susceptibility and treatment of bovine subclinical mastitis. Indian Veterinary Journal, 1993, vol. 3, no. 6, pp. 201–203.

15. Sozdillo Z. M., Doymaz M. Z, Oliver S. P. Morphological study of chronic Staph. aureus mastitis in the lactation bovine mammary gland. Res. In veter. Sc., 1989, vol. 47, no. 2, pp. 247–252.

16. Vachevsky S. S., Vachevskaya N. L., Budantsev A. I. Comparative characteristics of integrated cows with mastitis therapy. Actual problems of deseases of reproduction organs and breast gland in animals, 2005, WITH, pp. 45–48. (in Russian)

17. Vasilyev V. M. Prevention of mastitis in cows. Veterinary Medicine, 2004, no. 11, pp. 37–38. (in Russian)

Download full text in PDF format

© 2016 Institute of Animal Biology