Bìol. Tvarin, 2016, Volume 18, Issue 3, pp. 114–119



O. Jaremko

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Lviv national university of veterinary medicine and biotechnologies
named after S. Z. Gzhytskyj,
50 Pekarska str., Lviv 79010, Ukraine

For the aim to study the immune status of calves under the influence of pyridoxine hydrochloride six groups of newborn calves with 5 animals in each group were formed. The calves from control group received basic diet. The calves from the research groups from the first day of life in addition to the basic diet received different doses of pyridoxine hydrochloride: the I group — 1.0; the II — 2.0; the III — 3.0; the IV — 4.0 and the V group — 5.0 mg/kg of body weight. The blood for the examination was taken before the morning feeding on the 1st, the 5th, the 21st, the 60th and the 90th day after birth.

It was found that pyridoxine hydrochloride causes stimulation of lymphocytopoiesis by increasing the number of T- and B-lymphocytes. The minimal number of lymphocytes in the blood of calves of all groups was recorded on the first day of life. The stimulation of lymphocytopoiesis with pyridoxine hydrochloride was observed during 90 days and only in the last 30 days it slightly decreased. The maximal number of T-lymphocytes in the blood of calves was detected in the beginning and in the end of the experimental period; the minimal number of T-lymphocytes was recorded on the 21st day of experiment. The relative number of B-lymphocytes in the calves from the experimental groups was the lowest in the beginning of the experiment; it increased till 21st days of experiment and gradually decreased till the 90th day of experiment. The relative number of B-cells was lower in the research groups than in the control one but the absolute number of B-cells was the same or higher in the research groups during all postnatal periods. During the first 5 days of life the blood of calves was saturated with antibodies which are available in colostrum. Exogenous pyridoxine does not affect the number of antibodies in the blood of calves. Only at a change of diet to forage the number of antibodies increases and reaches the maximum concentration at the end of the milk period.

The optimal dose of daily supplement of vitamin B6 for correction of vitamin-supply in calves at age 1–21 days is 4 mg/kg of body weight, in calves at age 21–60 days — 3 mg/kg of body weight, and in calves at age 60–90 days it is 2 mg/kg of body weight.


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