Bìol. Tvarin, 2016, Volume 18, Issue 3, pp. 17–22

http://dx.doi.org/10.15407/animbiol18.03.017

INFLUENCE OF AFLATOXIN В1 ON PROOXIDANT-ANTIOXIDANT BALANCE IN CELLS OF WHITE RATS

N. K. Hoyvanovych

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Drohobych State Pedagogical University named after Ivan Franko,
23 T. Shevchenko str., Drohobych, Lviv region, 82100, Ukraine
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The article presents the results of the researches of influence of aflatoxin В1 (AFB1) on activity of enzymes of the antioxidant system (glutathione peroxidase, glutathione reductase and glutathione-S-transferase) and content of renewed glutathione in the cells of organs (liver, brain, kidneys). The antioxidant system prevents accumulation of toxic products of an oxidization peroxide of lipids; it plays an important role in detoxication, degradation and leading foreign organic substances out from the organism.

The experimental rats during 14 days received the aflatoxin В1 in a dose 0,025 mg/kg of the body weight. In the investigated organs under the influence of the protracted intoxication the processes of decompensation of antiradical defence are activated. It is established that under the influence of intoxication with the aflatoxin В1 the antioxidant system plays an important role in reduction of toxic effects of the toxin. During the protracted intoxication the activity of enzymes of the antioxidant system (glutathione peroxidase, glutathione reductase, glutathione-S-transferase activity and glutathione content) is decreasing. The dynamics of glutathione reductase and glutathione peroxidase in the cells of rats which received AFB1 indicates the exhaustion of backlogs of the glutathione renewable biochemical system. When analysing functional activity of glutathione-S-transferase, it is important to mention that under the influence of daily application of aflatoxin to the organism of rats the activity of the enzyme is considerably decreased on 7th and 14th day of experiment. For certain, it takes place as a result of inactivation of molecules of the enzyme by binding them with products of biotransformation of aflatoxin.

The results of researches testify the development of the oxidative stress in the investigated cells under action of aflatoxin В1. At the same time, the mechanisms of pathogenic action of aflatoxins can result in induction of formation process of the free radicals and initiation of reactions of lipid peroxidation which causes the decrease of antioxidant defence. The received results testify the considerable changes of prooxidant-antioxidant balance of cells under the influence of the protracted intoxication by the aflatoxin В1.

Keywords: AFLATOXIN В1, ANTIOXIDANT SYSTEM, RENEWED GLUTATHIONE, GLUTATHIONE REDUCTASE, GLUTATHIONE PEROXIDASE, GLUTATHIONE-S-TRANSFERASE, LIVER, BRAIN, KIDNEYS

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