Bìol. Tvarin, 2016, Volume 18, Issue 2, pp. 119–124

http://dx.doi.org/10.15407/animbiol18.02.119

STATUS OF INDICATORS OF HUMORAL LINK OF NATURAL IMMUNITY IN RATS UNDER OXIDATIVE STRESS AND ACTION OF LIPOSOMAL PREPARATION

M. Khariv

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Lviv national university of veterinary medicine and biotechnologies named after S.Z.Gzhytsky,
50 Pekarska str., Lviv 79010, Ukraine

The article displays the results of the research of the influence of developed complex liposomal preparation on the dynamic indices of humoral links of natural resistance in rats. Under these conditions it was modeled an oxidative stress, which is caused by the use of carbon tetrachloromethane.

It was also proved that intramuscular injection of 50 % tetrachloromethane at a dose of 0.25 ml per 100 g of body weight applied to rats of the research groups determines the antigenic load on the body and increases the intensity of humoral link of natural resistance. This is indicated by the content increase of circulating immune complexes in serum throughout the period of research. On the 14th day the level of high-mentioned index was 93 mmol/l, which is 2.2 times more than the initial values of the control group. At the same time it was found a decrease of bactericidal activity and lysozyme activity of serum on the 10th and 14th day of research. On the 2nd day of research the increase of bactericidal activity of serum by 6.6 % and lisozyme activity of serum to 7.25 % was established and on the 14th day of experiment bactericidal activity in biological object of blood in rats of the 1st experimental group decreased by 8.18 % and lizosyme activity of serum — on 3.95 %.

To increase the humoral link of natural resistance of the rat organism at oxidative stress it is appropriate to use liposomal drug which contains butaphosphate, interferon, milk thistle and vitamins. After applying liposomal preparation to rats under conditions of oxidative stress during researches the normalization of humoral natural resistance occurs in the blood, namely there is no deviation from the normal indices of bactericidal and lizosyme activity in serum and the level of circulating immune complexes. On the 14th day of experiment the indices of humoral link of immune system in the blood of experimental rats ranged within physiological values.

Keywords: RATS, TETRACHLORMETANE, OXIDATIVE STRESS, BACTERICIDAL ACTIVITY, LIZOCIMIC ACTIVITY, LIPOSOMAL PREPARATION

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