Bìol. Tvarin, 2016, Volume 18, Issue 2, pp. 36–44

http://dx.doi.org/10.15407/animbiol18.02.036

EFFECT OF 5-HT4RECEPTORS AGONIST (MOSAPRIDECITRATE)ON LIPIDPEROXIDATION, NITROGEN (II) OXIDE PRODUCTION ANDMORPHOLOGYOF COLON MUCOSA UNDER THE CONDITIONS OF ULCERATIVE COLITIS

N. Denysenko, A. Sklyarov

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Danylo Halytskyy Lviv National Medical University,
69 Pekarska str., Lviv 79010, Ukraine, zimenkovsky@meduniv.lviv.ua

Serotonin plays an important role in function regulation of the gastrointestinal tract, including the colon. We know that almost all of human body’s serotonin is synthesized and deposited in enterochromaffin cells localized in colon mucosa. Implementation of serotonin influence on smooth muscles, blood vessels and colon mucosa is performed by activating various types of serotonin receptors. 5-HT4receptors play significant role among them as they are identified on cell membranes of the colon (enterocytes, smooth muscle, endothelium).

We used mosapride citrate a 5-HT4 receptors agonistto determine their role. The study was conducted in nonlinear rats of both sexes, who were divided into 4 groups (first — intact animals,second — animals, injected 5-HT4receptors agonist, third — animals with experimental colitis, fourth — animals injected 5-HT4receptors agonist under condition of experimental colitis). Colitis was modeled by introducing a solution of acetic acid in the colon.

The colitis was accompanied by increased activity of lipid peroxidation processes, inducible NO-synthase, myeloperoxidase, which causes the development of nitrozo-oxidative stress. Mosapride action does not lead to severe morphological and biochemical changes in the colon mucosa. Activation of 5-HT4 receptors by mosaprideunder the condition of colitis reduced degree of destructive damage, content of TBA-active products, the activity of myeloperoxidase, inducible NO-synthase, superoxide dismutase and catalase in the colon mucosa, indicating a decrease of nitrozo-oxidative stress and strengthening of cytoprotection. Application of mosapride citrate has not only prokinetic effect, but also cytoprotective, antioxidant and anti-inflammatory under condition of experimental colitis.

Keywords: EXPERIMENTAL COLITIS, 5-НТ4 RECEPTORS, NO-SYNTHASE, LIPID PEROXIDATION, ANTIOXIDANT ENZYMES

 
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