Bìol. Tvarin, 2016, volume 18, issue 2, pp. 9–17

STATE OF ANTIOXIDANT DEFENCE SYSTEM OF LOACH EMBRYOSAT INFLUENCE OF FLURENIZIDE

N. О. Bodnarchuk, SMMandzynets, LIPetrukh, DISanagurski

nataljabodnarchyk@ukr.net

Ivan Franko National University of Lviv,
4 Hrushevskogo
str., Lviv 79005, Ukraine, This email address is being protected from spambots. You need JavaScript enabled to view it.

The aim of this work was to study the influence of Flurenizide (antibiotic of antimicrobial, antituberculous, antichlamydia, immunomodulator, antioxidant, hepatoprotective, antiinflammatory, antiviral action) on the antioxidant homoeostasis of loach embryos (Misgurnus fossilis L.) during early embryogenesis.The superoxide dismutase and catalase activity under the action of Flurenizide in concentrations 0,01; 0,05; 0,15; 1; 5; 15 mM of loach embryos at the stage of development first (2 blastodmeres), fourth (16 blastodmeres), sixth (64 blastodmeres), eighth (256 blastodmeres), tenth (1024 blastodmeres) crushing zygote was investigated. The twofactor analysis of variance was conducted to expose the level of influence of Flurenizide action, time of development and untaken into account factors on activity of the investigated enzymes.

It is established that Flurenizide violates the work of superoxiddysmutase on all stages of loach embryos development. It predetermines the slump of activity of this enzyme on the stage of development 16 blastodmeres. It is revealed that the investigated antibiotic in concentrations 1; 5; 15 mМ on the stage of the 10th division of bioblasts predetermines the slump of activity of superoxiddysmutase, while in the concentrations of 0,01mМ and 0,05 mМ Flurenizide causes the increase of this enzyme activity.

It is validified that Flurenizide in all investigated concentrations causes the slump of catalase activity on the stage of 64 blastodmeres of loach embryos development. On the stage of the 10th loach embryos division Misgurnus fossilis L. Flurenizide in the concentrations of 0,01 mМ and 0,05 mМ predetermines the considerable increase of catalase activity and in the higher concentrations of 1 mМ and 15 mМ conduces to the slump of activity of the investigated enzyme.

By means of twofactor analysis of variance it is established that the powerful influence on superoxide dismutase and catalase activity of loach the embryos is rendered by the untaken into account factors. Flurenizide carries out a mediocre influence on work of these enzymes which testifies probably the indirect action of this factor on activity of SOD and CAT. It is established that time of development has more expressed influence on catalase activity.

Keywords: LOACH EMBRYOS, FLURENIZIDE, EMBRYOGENY, SUPEROXIDE DISMUTASE, CATALASE

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